【摘要】 目的探讨丙泊酚对不同发育时期SD大鼠少突胶质细胞鞘磷脂蛋白(MBP)的影响。方法取3、7、14、21日龄SD大鼠各40只,随机分为对照组和实验组,每组20只。对照组(中长链脂肪乳组)和实验组(丙泊酚中长链脂肪乳注射液组)分别腹腔注射脂肪乳25 mg/kg或丙泊酚25 mg/kg,隔20 min追加首次剂量的1/2,持续8 h。通过荧光定量RT-q PCR法检测MBP mRNA和Caspase-3mRNA的表达,Western blot和免疫组化法检测MBP蛋白的表达。结果与对照组相比,实验组各日龄大鼠MBP mRNA的表达均明显下调(P<0.05),而3、7、14日龄大鼠Caspase-3mRNA表达明显上调(P<0.05)。实验组7、14日龄大鼠MBP的表达,与对照组相比显著降低(P<0.05)。两组21日龄Caspase-3mRNA和MBP的表达,差异没有统计学意义(P>0.05)。结论丙泊酚可抑制SD大鼠MBP基因与蛋白的表达,以7、14日龄最为显著。更多还原

【Abstract】 Objective To investigate the effect of propofol on myelin basic protein(MBP) expression in oligodendrocytes of SD rats at different developmental stages.Methods This study was conducted in 3-,7-,14-and 21-day-old SD rats(40 in each age group).In each group,the rats were randomized equally into control group and experimental group,and in the control group,the rats received an intraperitoneal injection of 25 mg/kg medium-long-chain fat emulsion followed by injections at a half dose every 20 min for 8 h; the rats in the experimental group were given injections of propofolmedium(at the initial dose of 25 mg/kg) in the same manner.The transcriptional levels of MBP and caspase-3 in the brain tissues were detected by q RT-PCR,and the protein expression of MBP was with Western blotting and immunehistochemistry.Results Compared with those in the control groups,the expression of MBP mRNA was significantly down-regulated while caspase-3 mRNA was up-regulated in3-,7-and 14-day-old rats in the experimental groups(P<0.05).The protein expression of MBP in 7-and 14-day-old rats was significantly decreased in the experimental groups compared with the control groups(P<0.05).The expression of caspase-3 mRNA or MBP protein in 21-day-old rats showed no significant difference between the two groups(P>0.05).Conclusion Propofol can down-regulate the expression of MBP at both the mRNA and protein levels in SD rats,especially in those at 7 and14 days of age.更多还原