【摘要】 目的建立全自动生化分析仪测定血清中碘含量的检测方法。方法取离心后的血清样品200μl,以高氯酸和氯酸钾为消解液,在130℃恒温下消解2 h。利用碘对砷铈氧化还原反应的催化作用,结合全自动生化分析仪的软硬件条件,测定血清中碘含量测定。开展了对本方法进行的线性范围、检测限、精密度、加标回收、方法比对等验证试验。结果在设定的仪器参数条件下,在血清碘浓度在0~300μg/L范围内,线性关系良好,平均相关系数的绝对值|r|达到了0.9998,方法检测限为0.435μg/L(取样量为200μl)。精密度:取低、中、高浓度血清碘样品,重复6次检测,相对标准偏差分别为2.5%、0.76%、0.64%。准确度:3种浓度血清样品加标回收率分别为106.6%、98.4%、94.3%,总平均回收率为99.8%。方法比对试验,应用全自动生化仪与砷铈催化分光光度法(国标法)各检测51份血清样本,经配对t检验,差异无统计学意义(t=0.51,P>0.05);与国标法相比,全自动生化分析仪法亚砷酸的使用量降为其5.3%(mg/份:0.4∶7.5)。结论应用全自动生化分析实现血清碘含量的定量测定,使用自配标准试剂,减低检测成本;自动化的加样、加试剂方式与恒温水浴装置,对人员操作和环境要求低,减少了检测过程中检验人员接触易感样品和剧毒试剂的机会,极大地降低了亚砷酸使用量,降低了对检测人员的危害和环境的污染。此方法适用于大批量的血清样品的碘含量测定。更多还原

【Abstract】 Objective To establish a method for the determination of iodine content in serum by automatic biochemical analyzer.Methods The serum samples after centrifugation were taken 200 μl, with perchloric acid and potassium chlorate as digestion solution, and at 130 ℃ at constant temperature for 2 hours. The determination of iodine content in serum was determined by the catalytic action of iodine on the redox reaction of arsenic and cerium, combined with the hardware and software conditions of the automatic biochemical analyzer. The linear range, the detection limit, the precision, the recovery of the addition standard are carried out, and the test of the method is compared with the equivalent verification test.Results Under the set of instrument parameters, the linear relationship between iodine concentration in the range of 0 ~ 300 μg/L was well, and the absolute value of the average correlation coefficient reached 0.9998. The detection limit of the method was 0.435 μg/L( the sampling volume was 200μl). Precision: the samples of low, medium and high concentration of serum iodine were taken and repeated 6 times.The relative standard deviations were 2.5 %,0.76 % and 0.64 % respectively. Accuracy: the recovery rates of the 3 concentrations of serum samples were 106.6 %,98.4 % and 94.3 % respectively, and the total average recovery rate was 99.8 %.The method of comparative tests,using automatic biochemical analyzer and arsenic cerium catalytic spectrophotometry( GB) detection of 51 serum samples,paired t test, the difference was not statistically significant( t = 0.51,P >0.05); compared with the standard method, using automatic biochemical analyzer, reduced to 5.3 %( the arsenate mg: 0.4 vs 7.5).Conclusion The quantitative determination by automatic biochemical analysis of serum iodine content, using self-made standard reagent, reduce the detection cost; automatic sampling, reagent adding method and device of constant temperature water bath, operation and low environmental requirements,avoid susceptible samples and poisonous reagent inspection personnel in the process of contact, which greatly reduces the sub arsenate usage, reduce the testing personnel hazards and environmental pollution. This method is suitable for the determination of iodine content in large quantities of serum samples.更多还原