【摘要】 本试验旨在研究金雀异黄素(genistein,GEN)对雌性大鼠体内促性腺激素及胰岛素样生长因子表达的影响。选取40只SD雌性大鼠[体重(200±20)g],随机分为5组,分别为阴性对照(NC)组、GEN低(L)、中(M)、高剂量(H)组及阳性对照(PC)组,每组8只,NC组灌胃花生油(其他组灌胃试剂以此为溶剂);L、M、H组分别灌胃15、30、60 mg/(kg BW·d)GEN,PC组灌胃己烯雌酚0.5 mg/(kg BW·d)。试验期30 d。采用酶联免疫吸附试验(ELISA)法检测血清中卵泡刺激素(FSH)、黄体生成素(LH)、胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-1(IGFBP-1)含量;实时定量PCR法检测卵巢IGF-1、IGFBP-1 mRNA表达水平。结果表明:与NC组比较,试验组血清中FSH、LH含量有升高趋势,但差异不显著(P>0.05),作用效果与PC组一致;试验组血清IGF-1含量略有降低,但差异不显著(P>0.05),PC组显著降低(P<0.05);试验组血清IGFBP-1含量显著或极显著升高(P<0.05或P<0.01),PC组显著升高(P<0.05);试验组卵巢组织中IGF-1、IGFBP-1 mRNA表达水平均升高,其中M、H组显著升高(P<0.05),与PC组变化一致。由此可见,GEN能够提高雌性大鼠血清FSH、LH含量、降低血清IGF-1含量、提高血清IGFBP-1含量,同时提高卵巢中IG FBP-1、IG F-1 mRNA的表达水平,这些指标协同作用于卵巢,能够促进卵泡的成熟,调节卵巢功能。更多还原

【Abstract】 In order to study the effects of genistein( GEN) on expressions of gonadotropins and insulin-like grow th factors in female rats,forty female SD rats [( 200 ± 20) g]w ere selected,and randomly divided into 5 groups w ith 8 rats per group,w hich w ere negative control( NC) group,GEN low dose( L) group,GEN middle dose( M) group,GEN high dose( H) group,positive control( PC) group. Rats in NC group w ere intragastric administrated peanut oil( other groups used it as solvent),those in L,M and H groups w ere intragastric administrated GEN at 15,30 and 60 mg/( kg BW ·d),and those in PC group w ere intragastric administrated diethylstilbestrol at 0.5 mg/( kg BW ·d). The experiment lasted for 30 days. Serum contents of follicle-stimulating hormone( FSH),luteinizing hormone( LH),insulin-like grow th factor-1( IGF-1) and insulin-like grow th factor binding protein-1( IGFBP-1) w ere detected by enzyme linked immunosorbent assay( ELISA) method. The mRNA expression levels of IGF-1 and IGFBP-1 in ovary tissue were detected by realtime quantitative PCR. The results show ed as follow s: compared w ith NC group,serum contents of FSH and LH in experimental groups tended to be increased,but the differences w ere not significant( P > 0. 05),w hich show ed the same tendency w ith those in PC group; serum content of IGF-1 in experimental group w as slightly decreased,but the difference w as not significant( P> 0.05),and that in PC group w as significantly decreased( P<0.05); serum content of IGFBP-1 in experimental groups was significantly increased( P < 0. 05 or P <0.01),and that in PC group w as significantly increased( P < 0. 05); mRNA expression levels of IG F-1 and IG FBP-1 in ovary tissue w ere increased,especially w ere significantly increased in M and H groups( P < 0.05),w hich show ed the same tendency w ith those in PC group. It is concluded that GEN can increase serum contents of FSH and LH,decrease serum IGF-1 content,and increase serum IGFBP-1 content,as w ell as increase mRNA expression levels of IG FBP-1 and IG F-1 in ovary of rats. These indexes synergistically affect ovary,can promote follicle maturation and improve the function of ovary.[Chinese Journal of Animal Nutrition,2017,29( 10) : 3604-3610]更多还原