【摘要】 本试验旨在研究枯草芽孢杆菌(BS)048对雪山草鸡抗肠炎沙门氏菌(SE)感染能力的影响。选择240只体重(61.5±0.5)g的1日龄雪山草鸡公鸡(SE阴性),随机分为4组,每组设3个重复,每个重复20只鸡。对照组和对照感染组饲喂基础饲粮,BS组和BS感染组饲喂添加0.1%(质量分数)BS048的基础饲粮。对照感染组和BS感染组在5~7日龄时每天以1×108CFU的SE感染试验鸡只,试验期14 d。测定BS048对正常和SE感染雪山草鸡盲肠黏膜、肝脏、肾脏SE载菌量,血清免疫球蛋白(Ig)和回肠分泌型免疫球蛋白A(s Ig A)含量,空肠黏膜碱性磷酸酶(ALP)、髓过氧化物酶(MPO)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性与总抗氧化能力(T-AOC)以及丙二醛(MDA)含量的影响。结果表明:1)与对照组相比,BS组显著提高了雪山草鸡8、10、14日龄回肠s Ig A含量(P<0.05),显著提高了10、14日龄空肠黏膜ALP活性(P<0.05),极显著提高了10、14日龄的空肠黏膜T-AOC及T-SOD、GSH-Px活性(P<0.01),显著降低了8、14日龄的空肠黏膜MDA含量(P<0.05)。2)与对照感染组相比,BS感染组极显著减少了SE感染雪山草鸡盲肠黏膜、肝脏和肾脏SE载菌量(P<0.01),显著提高了10、14日龄的血清Ig A、Ig G、Ig M及8、10、14日龄的回肠s Ig A含量(P<0.05),显著提高了8、10、14日龄的空肠黏膜ALP活性P<0.05),极显著降低了8、10、14日龄的空肠黏膜MPO活性(P<0.01),显著或极显著提高了8、10、14日龄的空肠黏膜T-AOC及T-SOD、GSH-Px活性(P<0.05或P<0.01),显著或极显著降低了8、10、14日龄的空肠黏膜MDA含量(P<0.05或P<0.01)。由此可见,BS048能够增强雪山草鸡的抗SE感染能力。更多还原

【Abstract】 The aim of this study was conducted to investigate the effects of Bacillus subtilis 048(BS048) on the anti-Salmonella enteritis(SE) infection ability of Xueshan chickens.Two hundred and fourty 1-day-old SE-negative Xueshan cocks with similar weight of(61.5±0.5) g were randomly divided into four groups with3 replicates per group and 20 chickens per replicate.Chickens in the control group and control infected group were fed a basal diet,and the others in the BS group and BS infected group were fed the basal diet supplemented with 0.1%(mass fraction) BS048.The experiment lasted for 14 days.The control infected group and BS infected group were infected with 1×10~8 CFU SE per cock each day for 3 days when they were 5-day-old,and to observe the effects of the BS048 on the SE amount in cecal mucosa,liver and kidney,serum immunoglobulin(Ig) and ileal secretory immunoglobulin A(slgA) contents,and alkaline phosphatase(ALP),myeloperoxidase(MPO),total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px) activities and total antioxidant capacity(T-AOC) and malonaldehyde(MDA) content in jejunum mucosa of normal and SE infected Xueshan chickens.The results showed as follows:1) compared with the control group,BS group significantly increased the ileal sIgA content of Xueshan chickens at 8,10 and 14 days of age(P<0.05),and significantly increased the activity of ALP in jejunum mucosa at 10 and 14 days of age(P<0.05),and significantly increased the T-AOC and activity of T-SOD and GSH-Px in jejunum mucosa at 10 and 14 days of age(P<0.01),while significantly decreased the jejunum mucosa MDA content at 10 and 14 days of age(P<0.05).2) Compared with the control infected group,BS infected group significantly reduced the SE amount in cecal mucosa,liver and kidney of SE infected Xueshan chickens(P<0.01),significantly increased the contents of IgA,IgG and IgM in saerum at 10 and 14 days of age and sIgA in ileum at 8,10 and 14 days of age(P<0.05),significantly increased the jejunum mucosa ALP activity at 8,10 and 14 days of age(P<0.05),significantly decreased the jejunum mucosa MPO activity at 8,10 and 14 days of age(P<0.01),significantly increased the T-AOC and activity of T-SOD and GSH-Px in jejunum mucosa at 8,10 and 14 days of age(P<0.05 or P<0.01),while significantly decreased the jejunum mucosa MDA content at 8,10 and 14 days of age(P<0.05 or P<0.01).It is concluded that S048 can enhance the anti-SE infection ability of Xueshan chickens.更多还原