In situ Crosslinked Hydrogel as Drug Carrier for Promoting Angiogenesis

【摘要】 Hydrogels composed of natural polysaccharides hold great potential as a release system for delivery of small molecule drugs.In this study,the functional drug sphingosine 1-phosphate(S1P)was loaded in the hydrogel which was derived from aldehyde hyaluronic acid(AHA)and carboxymethyl chitosan(CC)through Schiff’s base reaction for promoting angiogenesis.The scanning electron microscopy(SEM)images demonstrated the suitable threedimension network structure of hydrogel.The rheological properties and compressive stress-strain behavior of hydrogel were also examined,and the results indicated that the hydrogel possessed good mechanical properties.In vitro drug release behaviors showed that drug released from the hydrogel in a sustained manner and could achieve prolonged release time compared to the pure drug.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraoliumbromide(MTT)assay showed the good cytocompatibility of the resulting hydrogel.More importantly,the chicken chorioallantoic membrane(CAM)assay confirmed that the S1P loaded hydrogel could significantly promote angiogenesis in the CAM model.As a result,our results strongly suggested that the as-prepared hydrogel would be a good candidate for delivery of functional drugs.更多还原

【Abstract】 Hydrogels composed of natural polysaccharides hold great potential as a release system for delivery of small molecule drugs.In this study,the functional drug sphingosine 1-phosphate(S1P)was loaded in the hydrogel which was derived from aldehyde hyaluronic acid(AHA)and carboxymethyl chitosan(CC)through Schiff’s base reaction for promoting angiogenesis.The scanning electron microscopy(SEM)images demonstrated the suitable threedimension network structure of hydrogel.The rheological properties and compressive stress-strain behavior of hydrogel were also examined,and the results indicated that the hydrogel possessed good mechanical properties.In vitro drug release behaviors showed that drug released from the hydrogel in a sustained manner and could achieve prolonged release time compared to the pure drug.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraoliumbromide(MTT)assay showed the good cytocompatibility of the resulting hydrogel.More importantly,the chicken chorioallantoic membrane(CAM)assay confirmed that the S1P loaded hydrogel could significantly promote angiogenesis in the CAM model.As a result,our results strongly suggested that the as-prepared hydrogel would be a good candidate for delivery of functional drugs.更多还原