We developed a fast in vitro propagation system for Ilex verticillata with the segments of tender stems as explants. We tested various culture media for inducing axillary buds and roots and for shoot multiplication,and studied the influences of subculture times and period on multiplication were studied. The results show that B5+6-BA1.0 mg·L~(-1)+IBA 0.05 mg·L~(-1) was the optimal culture medium for inducing axillary buds. The optimal culture medium for shoot proliferation was B5+6-BA 1.5 mg·L~(-1)+IBA0.3 mg...