【摘要】 目的:探讨子宫内膜异位症患者在位及异位内膜胰岛素样生长因子-1(IGF-1)及胰岛素样因子结合蛋白-3(IGFBP-3)的表达水平,并探讨IGF-1及IGFBP-3在子宫内膜异位症发病过程中的作用,为临床治疗子宫内膜异位症提供新的思路。方法:选择子宫内膜异位症患者共64例(内异症组),另选择同期入院的非子宫内膜异位症患者54例作为对照(非内异症组),2组患者均经手术治疗,术毕分别取2组患者的内膜组织,经免疫组化、染色等步骤,制作好切片,在光镜下进行结果判定。结果:免疫组化结果显示,IGF-1在内异症患者的异位内膜间质细胞和腺上皮细胞均呈中度、强阳性表达,在内异症患者的在位内膜间质细胞(59例)及腺上皮细胞(62例)呈中度、强阳性;在非内异症患者子宫内膜间质细胞(48例)及腺上皮细胞(52例)呈阴性表达;IGFBP-3在内异症患者异位内膜间质细胞(62例)及腺上皮细胞(60例)呈阴性表达,在内异症患者在位内膜间质细胞(58例)及腺上皮细胞(52例)呈阴性表达,在非内异症患者子宫内膜间质细胞(51例)及腺上皮细胞(48例)呈强阳性表达。结论:IGF-1在子宫内膜异位症患者异位内膜及在位内膜均呈中度阳性及强阳性表达,在非内异症患者子宫内膜细胞则大部分呈阴性表达;而IGFBP-3在子宫内膜异位症患者异位内膜及在位内膜大多数均呈阴性表达,在非内异症患者子宫内膜细胞大多数呈阳性表达,推测IGF-1在子宫内膜异位症发病过程中起了一定的作用,而IGFBP-3在对抗子宫内膜异位症的发生发展中有一定作用。更多还原

【Abstract】 Objective:To investigate the expression levels of ectopic endometrial insulin-like growth factor-1(IGF-1)and insulin-like factor-binding protein-3(IGFBP-3)in patients with endometriosis,and to explore the relationship between insulin-like growth factor-1 and insulin-like factor-binding protein-3 in the process of endometriosis,then to provide new ideas for the clinical treatment of endometriosis.Methods:A total of 64 patients with endometriosis(endometriosis group)were selected from.And 54 patients with non-endometriosis admitted in the same period were selected as control group(nonendometriosis group).The two groups of patients were surgically treated.After surgery,the endometrial tissues of the two groups were taken respectively.The sections were made by immunohistochemistry,staining and other steps,then the resuts were determined in the light microscope.Results:The results of immunohistochemistry showed that IGF-1 expressed moderately and strongly positive in ectopic endometrial stromal cells and glandular epithelial cells in patients with endometriosis,and the eutopic endometrial stromal cells(59 cases)and glandular epithelial cells(62 cases)were moderately and strongly positive;In non-endometriosis patients,endometrial stromal cells(48 cases)and glandular epithelial cells(52 cases)were negative expression;IGFBP-3(28 cases)and glandular epithelial cells(52 cases)were negative expression in endometrial stromal cells(58 cases)and glandular epithelial cells(52 cases)in patients with endometriosis Negative expression of endometrial stromal cells(51 cases)and glandular epithelial cells(48 cases)in non-endometriosis patients were strongly positive expression.Conclusion:IGF-1 expression was moderately positive and strongly positive in ectopic endometrium and eutopic endometrium of patients with endometriosis.Most of the endometrial cells in non-endometriosis patients are negative expression;IGFBP-3 expression in ectopic endometriosis and eutopic endometrium were mostly negative in non-endometriosis patients.Therefore,we can conclude that IGF-1 plays a role in the pathogenesis of endometriosis while IGFBP-3 plays a role in the development of endometriosis.更多还原