【摘要】 目的研究氯苯丙氨酸(PCPA)诱导的失眠大鼠前额皮质三磷酸腺苷(ATP)不同时间水平变化情况,探讨酸枣仁汤(SZRD)的干预机制。方法大鼠分为正常对照组(不做任何操作),SZRD对照组(第1、2、3天腹腔注射蒸馏水,第4~10天蒸馏水灌胃),空白对照组,模型组,SZRD治疗组,五羟色氨酸(5-HTP)组,5-HTP+SZRD组。采用连续3d腹腔注射PCPA的方法建立大鼠失眠模型,各组在实验的第4、5、6、8、10天分别取大鼠前额叶皮质,采用高效液相色谱法(HPLC)检测各组大鼠不同时间前额皮质ATP水平。结果模型组大鼠实验第4、5、6、8、10天ATP水平显著下降,与空白对照组比较差异有统计学意义(P<0.01);SZRD治疗组第4、5、6、8、10天ATP水平较模型组逐渐升高,差异有统计学意义(P<0.05,P<0.01);SZRD对照组前额叶皮质ATP水平与空白对照组比较差异无统计学意义(P>0.05);空白对照组和正常对照组比较,前额皮质ATP水平差异无统计学意义(P>0.05);5-HTP组与SZRD对照组、5-HTP+SZRD组之间比较差异也无统计学意义(P>0.05)。结论 SZRD与5-HTP本身对正常大鼠前额皮质ATP水平变化无显著影响,SZRD对前额皮质ATP的作用出现在PCPA所致失眠后。更多还原

【Abstract】 Objective The study is intended to examine changes in prefrontal cortex ATP concentrations over time in PCPA induced insomnia rats,and to examine the intervention effects of Suanzaoren decoction.Methods SD rats randomly assigned to normal control,control,model,SZRD control,SZRD treatment,5-HTP,and 5-HTP + SZRD groups.PCPA was injected intraperitoneally over three days to induce the insomnia model.One day after induction,at the fourth,fifth,sixth,eighth,and tenth days after experiment,subjects from each group were examined for prefrontal cortex ATP concentration using the HPLC method.Results Prefrontal cortex ATP concentrations in the PCPA Model group at the fourth,fifth,sixth,eighth,and tenth days were declined significantly compared with the Control group(P<0.01).Compared with the PCPA Model group,the SZRD treatment group showed significant(P<0.05)increase at the fourth,fifth,sixth,and eighth days after assay,and a significant increase(P<0.01)on the tenth day.A comparison of the Control group with the Normal control group,and a comparison among the SZRD control group,the5-HTP,and 5-HTP+SZRD groups showed no significant differences in prefrontal cortex ATP concentrations.Conclusion SZRD with 5-HTP itself has no significant effect on normal rats prefrontal cortex ATP levels,SZRD role of the prefrontal cortex appear to the ATP after PCPA caused insomnia.更多还原