【摘要】 目的采用高效液相色谱法(HPLC)体外模拟体内环境条件确定丙泊酚、依托咪酯混合后的新物质生成情况。方法采用十八烷基硅烷键合硅胶作为填充剂(250.0mm×4.6mm,5μm),以磷酸盐缓冲液-乙腈(调至pH7.4)作为流动相[磷酸盐缓冲液∶乙腈=35∶65(V/V)],设定柱温为30℃,流速为1.0mL/min,进样量为10μL,全波长下进行紫外光谱检测(波长190~800nm),通过对比标准品及对照品图谱确认图谱中是否有新的特征峰。结果通过与标准品图谱进行比较发现,丙泊酚、依托咪酯二者混合品在体外模拟体内环境条件下无新的特征峰出现,即未生成第3种物质。结论所建方法简便、快速、准确地检测出丙泊酚、依托咪酯混合后无新物质生成。更多还原

【Abstract】 Objective To establish a high performance liquid chromatography(HPLC)method for determinating the related substances of propofol and etomidate after mixing.Methods With octadecylsilane chemically bonded silica as the filler(250mm×4.6mm,5μm),the mixture of phosphate buffer-acetonitrile(phosphate buffer:acetonitrile=35:65(V/V))was acted as the mobile phase,and adjusting PH to 7.4.The UV detection wavelength:190-800 nm.The column temperature:30 ℃.The flow rate:1.0mL/min.The injection volume:10μL.The comparison result of standard substance and reference substance were used to confirm whether there was a new sabstance in the spectrum.Results There was no new substance generated.Conclusion There was no new substance generated of propofol and etomidate mixture by this simple,rapid and accurate method.更多还原