【摘要】 分别采用高功率脉冲磁控溅射（HPPMS）技术和直流磁控溅射（DCMS）技术在单晶硅基片上制备出两种晶粒尺寸的TiO₂纳米晶薄膜;采用ISO10993-5:1999标准中的浸提液法对TiO₂薄膜的体外细胞毒性进行评价和研究。实验结果表明,在TiO₂纳米晶薄膜浸提液中细胞生长相对增殖率（RGR）为93%⁹6%,细胞毒性评级为1级,TiO₂纳米晶薄膜作为生物医学材料满足临床应用的要求;对两种晶粒尺寸的薄膜进行体外细胞毒性的评级,结果无差异。更多还原

【Abstract】 In this paper,TiO₂ nanocrystalline films with two grain sizes were fabricated on the single-crystal silicon substrate by highpower pulsed magnetron sputtering（ HPPMS） and DC magnetron sputtering（ DCMS） respectively. The vitro cytotoxicity of TiO₂ nanocrystalline films were evaluated by extract method described in ISO10993-5: 1999 standard. The results showed that the relative growth rate（ RGR）of the cells was 93%—96% in the extract,and the cytotoxicity was grade 1 which met the requirements of clinical application. There was no significant difference in vitro cytotoxicity for the films with two grain sizes.更多还原