【摘要】 建立了小鼠胚胎成纤维(NIH/3T3)细胞紫外辐射损伤的实验模型,考察小球藻类金属硫蛋白(Zn-MT-like)对NIH/3T3细胞紫外辐射的保护作用。结果表明,小球藻Zn-MT-like对NIH/3T3细胞的生长无抑制作用,且具有较强的抗紫外辐射能力。质量浓度为10 g/L的小球藻Zn-MT-like可显著减少NIH/3T3细胞空泡、降低细胞裂解的程度,可使经紫外辐射的NIH/3T3细胞保持较好的活性。更多还原

【Abstract】 A laboratory model for testing ultraviolet irradiation damage of fibroblast( NIH /3T3) of mouse embryo was established; and it was applied for examination of protective effect of Zine- binding metallothionein- like( Zn- MT- like) extracted from C. vulgaris for NIH/3T3 cells against ultraviolet irradiation. Results showed that the Zn- MT- like presents a strong protective effect for the NH /3T3 cells from irradiation damage. For example,the Zn- MT- like at mass concentration of 10 g / L can effectively reduce the NIH /3T3 cell cavity and cell fragmentation under the ultraviolet irradiation,and maintaining rather good cell activity.更多还原