In this work,we characterized the metabolic responses induced by Vibrio anguillarum and V.splendidus challenges towards hepatopancreas of Manila clam Ruditapes philippinarum using nuclear magnetic resonance(NMR)-based metabolomics.Metabolic responses showed that both V.anguillarum and V.splendidus induced disturbances in energy metabolism,osmotic regulation,protein biosynthesis and oxidative stress with different mechanisms.Three metabolites(glucose,glutamate and threonine)were altered in both V.anguillarum...