MWCNTs-PtN Ps nano composite was prepared by ultrasonic method,and its modified to ethanol biosensor showed good performance. The experimental results show that the minimum detection limit of the sensor is 0. 02 mmol / L,the linear range is 0.25 mmol / L ~ 3.0 mmol / L,the sensitivity is 0.923 32 μA /( mmol / L),and has high stability and good reproducibility.