【摘要】 通过植物基因工程手段培育转基因作物品种,可改善作物的生存能力和对生态环境的耐受力。本研究以普那菊苣叶片（Cichorium intybus L.‘Puna’）为受体材料,用农杆菌介导方法,将来自水稻的由CaMV35S启动子驱动的生育酚环化酶（tocopherol cyclase,OsVTE）基因导入普那菊苣。经过卡那霉素（Km）筛选以及对抗性植株PCR和Southern杂交分析,获得了转基因植株。用不同浓度的蔗糖、琼脂和甘露醇溶液研究普那菊苣幼苗的耐旱性,发现转基因植株的耐旱性明显得到提高。蔗糖浓度为50g·L^-1、琼脂浓度为10g·L^-1和甘露醇浓度为30g·L^-1胁迫下普那菊苣的生理指标测定显示,转基因普那菊苣幼苗SOD、POD活性比未转化植株高2³倍,转基因普那菊苣幼苗MDA含量降低。本研究获得了稳定的转OsVTE基因植株,旱胁迫下的转基因植株的SOD酶活性和POD酶活性含量提高,表现出一定的耐旱性。更多还原

【Abstract】 Plant genetic engineering was widely used to improve crop viability and tolerance to environmental stresses.In this report,an OsVTEgene was introduced into Cichorium intybus L.‘Puna’by Agrobacterium-mediation transformtion.Transgenic chicory plants were obtained under Kanamycin selection,and confirmed by PCR and Southern-blot tests.Effects of different concentrations of sucroses,agarose and mannitol were used as a mimic of drought stress codiction to investigate drought tolerance of chicory seedlings.The results showed that drought tolerance of transgenic plants was better than those non-transgenic ones.Activity of peroxidase（POD）and superocide（SOD）dismutase of transgenic seedings at the sucrose concentration of 50 g·L^-1,agarose concentration of 10 g·L^-1 and mannitol concentration of 30 g·L^-1 was 2³ times higher than that of non-transgenic ones.Malonaldehyde（MDA）content in transgenic seedlings was lower than that of non-transgenci ones.Overall,stable transgenic plants with OsVTE were obtained.The transgenic plants showed higher drought tolerance,content of POD and SOD dismutase in transgenic plants was increased under drought stress conditions.更多还原