【摘要】 通过发芽试验,探讨不同浓度五氨基乙酰丙酸（5-ALA）对Cd胁迫、盐胁迫及干旱胁迫下草地早熟禾（Poa pratensis）种子萌发及幼苗生长的影响。本试验以草地早熟禾‘午夜’（Poa pratensis‘Midnight’）品种为材料,在Cd(2mg·L^-1),NaCl(50 mmol·L^-1),PEG（15%）3种处理下分别施加不同浓度的5-ALA(0,5,25,50,100mg·L^-1),分析了不同处理下种子发芽势、发芽率、可溶性蛋白等指标。结果表明:不同胁迫下较低浓度(5,25mg·L^-1)5-ALA均能提高种子的发芽势、发芽率、芽长、根长等指标,而较高浓度(50,100mg·L^-1)的5-ALA起抑制作用。综合比较4种不同浓度的5-ALA发现,25mg·L^-1对胁迫的缓解效应最优,其Cd胁迫下发芽势、发芽率分别比对照增加了21%,33.67%;NaCl胁迫下发芽势、发芽率分别增加了22.67%,23.67%;PEG模拟干旱胁迫下发芽势、发芽率分别增加了15.33%,10.67%。25mg·L^-1 5-ALA对NaCl,PEG胁迫下可溶性蛋白含量提高显著,分别提高了6.5和3.8μg·g-1,对于Cd胁迫,可溶性蛋白含量虽有提升但不显著。更多还原

【Abstract】 This trial was aimed to investigate the effects of different concentrations of 5-aminolevulinic acid（5-ALA）on seed germination and seedling growth of Poa pratensis under Cd stress,salt stress and drought stress with germination test.Poa pratensis ‘Midnight’was used as the plant material,and five different concentrations of 5-ALA(0,5,25,50,100 mg·L^-1)were applied to the treatment of Cd(2 mg·L^-1),NaCl(50 mmol·L^-1)and PEG（15%）.Germination energy,germination rate,soluble protein and other indexes were analyzed.The results showed that the germination energy,germination rates,bud length and root length increased with the lower concentrations(5,25 mg·L^-1)of 5-ALA,and they were inhibited at the higher concentrations(5,100 mg·L^-1).The best concentration was 25 mg·L^-1 after a general comparison among five concentrations of 5-ALA.Under this concentration,germination energy and germination rates were increased by 21%and 33.67%respectively compared with the control under Cd stress.Under salt stress,germination energy and germination rates were increased by 22.67% and23.67%respectively,and the two indexes were increased by 15.33% and 10.67% respectively under drought stress.In addition,soluble protein content was significantly increased by 6.5μg·g-1,3.8μg·g-1 when the seedling was under salt or drought stress.As for Cd stress,soluble protein content was improved,but the influence was not significant.更多还原