【摘要】 目的:观察印度獐牙菜对四氯化碳(CCl4)所致大鼠肝纤维化的防治作用。方法:32只SD大鼠随机分为空白组、模型组、印度獐牙菜组、阳性药组。除空白组外,各组大鼠腹腔注射40%CCl4/橄榄油溶液2m L/kg,每周2次,连续7周诱导肝纤维化。造模同时,给药组大鼠灌胃相应药物干预8周。实验结束后,观察肝组织病理学变化;测定血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅳ型胶原蛋白(ColⅣ)水平以及丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆红素(TBIL)含量。结果:与空白组比较,模型组可见明显肝纤维化病理改变,AST、ALT、ALP和TBIL水平明显升高(P<0.05),HA、LN、PCⅢ和ColⅣ含量显著增高(P<0.01);与模型组比较,印度獐牙菜组肝纤维化病理程度减轻,肝功能指标改善明显,ALT、TBIL水平显著降低(P<0.05),AST、ALP水平呈下降趋势,HA、LN、PC及ColⅣ含量显著下降(P<0.01)。结论:印度獐牙菜具有较好的抗肝纤维化作用。更多还原

【Abstract】 Objective: To observe the effects of Swertia chirayita on hepatic fibrosis model rats induced by carbon tetrachloride(CCl4). Methods: SD rats were randomly divided into the normal group, the model group, the Swertia chirayita group, and the positive drug group. All the groups except the normal group were intraperitoneally injected with 40%CCl4/olive oil solution 2m L/kg, twice a week for 7 consecutive weeks to induce hepatic fibrosis model. At the same time, rats in the treatment groups were given intragastric administration and the corresponding drugs were intervened for 8 weeks. Pathological changes of liver tissue were observed at the end of the experiment; the levels of serum hyaluronic acid(HA), laminin(LN), type three procollagen(PCⅢ) and type Ⅳ collagen(ColⅣ), and the content of alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) and total bilirubin(TBIL) were detected. Results: Compared with the normal group, the pathological changes of liver fibrosis were obvious in the model group, and the levels of AST, ALT, ALP and TBIL were significantly increased(P<0.05), the contents of HA, LN, PC and ColⅣ increased significantly(P<0.01); compared with the model group, the pathological degree of liver fibrosis in Swertia chirayita group decreased, the liver function indexes improved obviously, and the levels of ALT and TBIL decreased significantly(P<0.05), the levels of AST and ALP showed a downward trend; the contents of HA, LN, PC and ColⅣ decreased significantly(P<0.01). Conclusion: Swertia chirayitahad reasonable effect on anti-hepatic fibrosis.更多还原