【摘要】 目的:建立通痹胶囊乙酸乙酯部位的HPLC色谱指纹图谱测定方法。方法:采用HPLC法,Thermo Syncronis C18色谱柱(250mm×4.6mm,5μm)、检测波长290nm、流速0.8m L/min、柱温30℃、进样量10μL进行梯度洗脱,对13批通痹胶囊的乙酸乙酯部位样品进行指纹图谱测定。结果:确定葛根素为内标物,建立了生物碱类成分的HPLC对照指纹图谱,标定了21个共有峰,确定了2个共有峰,基本确定了17个色谱峰的药材归属,占总共有峰数的80.95%。结论:该方法建立的指纹图谱灵敏度高,稳定性好,可为通痹胶囊的质量控制提供可靠依据。更多还原

【Abstract】 Objective: To establish an HPLC-fingerprint method of ethyl acetate elution part of Tongbi Capsule. Methods: A separation was performed on a Thermo Syncronis C18 chromatographic column(250mm×4.6mm, 5μm), with gradient elution. The detection wave length was at 290 nm, mobile phase was at flow rate of 0.8m L/min, column temperature was 30℃, injection volume was 10μL. Determine ethyl acetate parts of 13 batches of Tongbi Capsule. Results: Determine puerarin as internal standard, established the reference fingerprint of alkaloids, selected 21 peaks in common, 2 common peaks were confirmed, the ownership of 17 peaks was established, occupying 80.95% of total amount. Conclusion: The method to establish the fingerprint is high sensitivity and good stability, it can provide reliable basis of the quality control of Tongbi Capsule.更多还原