【摘要】 目的:研究丹酚酸B液晶纳米粒(Sal B-LCN)跨鼻黏膜吸收及跨血脑屏障(BBB)吸收情况。方法:利用在体大鼠鼻灌流模型模拟鼻腔吸收,利用体外MDCK-MDR1细胞模型模拟血脑屏障吸收,两者模拟了Sal BLCN经鼻黏膜吸收并跨血脑屏障吸收入脑的过程,探究液晶纳米递药系统对丹酚酸B(Sal B)的促吸收作用。结果:Sal B和Sal B-LCN的鼻腔吸收速率常数(K)分别为(1.64±0.19)×10-3/min和(3.75±0.26)×10-3/min,转运吸收表观渗透系数(Papp)分别为(0.544±0.035)×10-6cm/s和(0.922±0.059)×10-6cm/s,皆具有显著性差异(P<0.05)。结论:液晶纳米递药系统能够促进Sal B在大鼠鼻腔及脑部的吸收,为开发Sal B鼻用液晶纳米制剂提供了实验依据。更多还原

【Abstract】 Objective: To study the absorption of Sal B liquid cubic crystal nanoparticles(Sal B-LCN) by the nasal mucosa and the blood-brain barrier(BBB). Methods: Nasal absorption and BBB absorption were simulated by in situ nasal perfusion in rats and external MDCK-MDR1 cell model in vitro respectively, which simulated the absorption process of Sal B-LCN from nasal cavity into the brain through BBB, so that the promoting effects of cubic crystal nanometer drug delivery system on salvianolic acid B(Sal B) absorption can be explored. Results: It was showed that the Sal B and Sal B-LCN nasal absorption rate constant(K) were(1.64±0.19)×10-3/min and(3.75±0.26)×10-3/min respectively, transit absorption apparent permeability coefficient(Papp) were(0.544±0.035) ×10-6cm/s and(0.922±0.059) ×10-6cm/s respectively, and significant statistic differences were found(P<0.05). Conclusion: Cubic crystal nano drug delivery systems can promote the absorption of Sal B in nasal cavity and brain, which provides experimental basis in developing Sal B nasal liquid cubic crystal nanometer.更多还原