【摘要】 目的:检测结肠腺癌患者癌组织中β-tubulinⅢ的表达,探讨其临床意义。方法:收集结肠腺癌组织标本111例,根据β-tubulinⅢ的阳性细胞分布部位分为浸润前沿组(n=72例)和非浸润前沿组(n=39例),采用免疫组织化学法检测结肠腺癌患者癌组织中β-tubulinⅢ的阳性表达率,分析β-tubulinⅢ的表达与患者性别、年龄、肿瘤分化、临床分期、淋巴结转移、复发和死亡等临床病理特征之间的关系。结果:不同性别、淋巴结转移、临床分期、死亡和复发结肠腺癌患者间β-tubulinⅢ的阳性表达率比较差异无统计学意义(P>0.05)。浸润前沿组和非浸润前沿组患者癌组织中β-tubulinⅢ阳性表达率比较差异有统计学意义(χ~2=8.76,P=0.01)。低中分化病例多见于浸润前沿组,而高分化病例多见于非浸润前沿组(χ~2=6.88,P=0.03)。不同分化程度癌组织中β-tubulinⅢ的阳性表达率比较差异有统计学意义(χ~2=5.74,P=0.04)。非前沿组中有无淋巴结转移的患者癌组织中β-tubulinⅢ阳性表达率比较差异有统计学意义(χ~2=6.02,P=0.05)。免疫组织化学法染色,β-tubulinⅢ阳性表达细胞胞质呈黄色颗粒;与低分化组比较,高分化癌组织中β-tubulinⅢ阳性表达细胞明显增多。结论:结肠腺癌组织中β-tubulinⅢ表达与肿瘤的分化程度有关,高分化结肠腺癌组织多见于非浸润前沿组。非浸润前沿组患者β-tubulinⅢ表达与淋巴结转移有关。更多还原

【Abstract】 Objective:To detect the expression and clinical significance ofβ-tubulin Ⅲ in cancer tissue of the patients with colon adenocarcinoma,and to explore its clinical significance.Methods:A total of 111 colon adenocarcinoma tissue samples were obtained.According to the location ofβ-tubulin Ⅲ positive cells,all patients were divided into front group(n=72)and non-front group(n=39).The positive expression rate ofβ-tubulinⅢin the patients with colon adenocarcinoma was detected with immunohistochemistry.The correlations among the expression of β-tubulin Ⅲ and gender,age,tumor differentiation,clinical stage,lymph node metastasis,recurrence and death were analyzed.Results:The expression levels ofβ-tubulin Ⅲ had no significant differences between the patients with different gerder,age,lymph node metastasis,clinical stages,death and recurrence.The positive expression rates ofβ-tubulin in cancer tissue of the patients had significant difference between front and non-front groups(χ~2=8.76,P=0.01).Lowly-to-moderately differentiated tissue was more common in front group,and highly-differentiated tissue was more common in non-front group(χ~2=6.88,P=0.03).There were significant differences in the expression levels ofβ-tubulin Ⅲ between cancer tissues with different differentiation degrees(χ~2=5.74,P =0.04).In non-front group,lymph node metastasis was closely correlated with the expression ofβ-tubulinⅢ(χ~2=6.02,P=0.05).The results of immunohistochemical staining showed that theβ-tubulin Ⅲ positive-expressing cells were colored brown-yellow.The number of cells with positiveβ-tubulin Ⅲexpression was significantly increased in highly differentiated tissue compared with low-differentiated tissue.Conclusion:The expression ofβ-tubulinⅢis closely related to tumor differentiation in colon adenocarcinoma tissue.The highly differentiated colon adenocarcinoma tissue is more common in non-front group in which the expression ofβ-tubulinⅢis related to lymph node metastasis.更多还原