【摘要】 目的对比分析4℃冷藏保存血小板与22℃振荡保存血小板对体外血小板减少模型的纠正效果,为冷藏保存血小板临床应用提供实验室依据。方法采用血栓弹力图及血小板计数等指标,评价分析不同保存时间的4℃冷藏保存血小板和22℃振荡保存血小板对体外血小板减少模型的纠正效果。结果 4℃冷藏保存血小板和22℃振荡保存血小板在保存到d 1、3、5时,对相同血样制备的血小板减少模型进行纠正,血小板计数从(10-30)×10~9/L纠正到100×10~9/L以上,4℃冷藏保存血小板到7-14 d时,同样达到纠正目的。4℃冷藏保存10-14 d的血小板与22℃振荡保存d 5的血小板对血小板减少模型进行纠正,纠正后的TEG-MA值均在正常值范围内,达到纠正效果。结论4℃冷藏保存10-14 d的血小板与22℃振荡保存5 d的血小板对体外血小板减少模型进行纠正,从血小板计数到TEG-MA值均达到了纠正效果,佐证4℃冷藏保存10-14 d的血小板具有良好的止血效果。更多还原

【Abstract】 Objective To compare the corrective effects on platelets in storage at 4℃ and agitation at 22℃ on the in vitro thrombocytopenic mode in order to provide an experimental basis for the preservation of platelets for clinical application.Methods Thromboelastography and platelet count were used to evaluate the in vitro corrective effects on thrombocytopenic platelets stored at 4℃ and those agitated at 22℃ for various durations.Results Platelets stored at 4℃ and those agitated at22℃ for 1,3,and 5 days were corrected using thrombocytopenic platelets prepared using the same blood samples.The platelet counts were corrected from 10-30 × 109/L to greater than 100 ×109/L.Platelets that had been stored at 4℃ for 7-14 days achieved similar correction values.Platelets that had been stored at 4℃ for 10-14 days and those agitated at 22℃ for 5 days were corrected using thrombocytopenic platelets.Their corrected thromboelastography(TEG)-MA values were within the normal range,and thus the corrective effects were achieved.Conclusion Platelets stored at 4℃ for 10-14 days and those agitated at 22℃ for 5 days were corrected using the in vitro thrombocytopenia mode.Based on the platelet counts,the TEG-MA values showed that corrective effects were achieved,thus supporting the claim that platelets stored at 4℃ for 10-14 days could achieve good coagulation effects.更多还原