Purposes—To explore the best experimental conditions for determining the content of flavonoids with UV-vis spectrophotometry and to test the content of total flavonoids in Sarcandra glabra beverage according to the fact that flavonoids can react with Al~(3+)under certain condition to generate the colored complex.Methods—With the content of total flavonoids as analysis indicator,the experimental conditions for determining of flavonoids with UV-vis spectrophotometry were optimized by single factor experiment ...