【摘要】 以黄瓜根际土壤为试材,采用亚历山大硅酸盐细菌培养基从黄瓜根际土壤中分离筛选解钾菌,用原子吸收法测定菌株的解钾能力,选出解钾能力较强的菌株进行菌种鉴定,同时研究其对黄瓜幼苗生长的影响。结果表明:从黄瓜根际土壤中共分离获得9株细菌,其中PK1、PK3和PK7具有较强的解钾能力,液体培养96h后其解钾率分别为30.05%、33.78%和44.01%。依据菌株的形态学特征和生理生化特征鉴定,PK1和PK3为恶臭假单胞菌(Pseudomones putida),PK7为胶质类芽孢杆菌(Paenibacillus mucilaginosus)。盆栽试验结果表明,与对照相比,14d后PK1提高黄瓜的根长、株高、鲜质量和干质量分别为42.60%、10.45%、48.65%、33.01%,PK3分别对应提高65.26%、21.09%、83.78%和49.48%,PK7分别对应提高79.46%、44.26%、105.41%和113.03%。更多还原

【Abstract】 Rhizosphere soil of cucumber was used as test material,K solubilizing bacteria were isolated in Alexandrove medium.The ability of dissolving potassium of K solubilizing bacteria were determined with the atomic absorption spectrophotometer,then the strains with stronger K solubilizing bacteria were identified,meanwhile,the effects on the growth of cucumber seedling were studied.The results showed that nine K solubilizing bacterium strains were isolated from cucumber rhizosphere soil,in which PK1,PK3 and PK7 demonstrated strong potassium decomposing ability,and potassium decomposing rate of them cultivated in liquid medium for 96hour was 30.05%,33.78%and 44.01%,respectively.Based on the morphological and physiological and biochemical characterestics,PK1and PK3 were identified into Pseudomones putida,PK7 was Paenibacillus mucilaginosus.In pot experiment,after 14days PK1increased the root length,plant height as well as fresh weight and dry weight of cucumber seedling by 42.60%,10.45%,48.65%and 33.01%,PK3 increased the correspondents by 65.26%,21.09%,83.78% and 49.48%,and PK7 increased the correspondents by79.46%,44.26%,105.41% and 113.03%,respectively,compared with the control.更多还原