【摘要】 目的观察miRNA-135在调控激素非依赖性前列腺癌细胞增殖能力中的作用及其机制。方法利用微小核糖核酸(miRNA)基因芯片检测激素非依赖性前列腺癌和激素依赖性前列腺癌组织中miRNA-135的表达差异。经过体外转染miRNA-135后对DU145和PC3细胞进行MTT检测,了解细胞的增殖情况。利用miRNA-135靶基因预测软件miRwalk和miRanda预测,初筛miRNA-135调控的基因和相关通路。结果 miRNA基因芯片结果显示,激素依赖性前列腺癌组织中miRNA-135的表达量高于激素非依赖性前列腺癌组织(P﹤0.05);miRNA-135可以抑制DU145和PC3细胞的生长,尤其在第48 h和第72 h以后(P﹤0.05);转染miRNA-135后,前列腺癌细胞株DU145和PC3中STAT6的表达水平较转染NC的细胞株明显下调。结论 miRNA-135能够抑制激素非依赖性前列腺细胞DU145和PC3的增殖能力,有望成为前列腺癌治疗的新靶标。更多还原

【Abstract】 Objective To investigate the role of miRNA-135 in regulating the proliferation of hormone-independent prostate cancer cells and its mechanism. Method The difference in miRNA-135 expression between hormone-independent prostate cancer and hormone-dependent prostate cancer was detected by micro RNA(miRNA) microarray analysis.Transfected DU145 and PC3 cells with miRNA-135 in vitro, then analyzed by MTT to detect cell proliferation. miRNA-135 target–gene–prediction–software miRwalk and miRanda were adopted to preliminarily screen miRNA-135-regulated genes and their related pathways. Result miRNA microarray results showed that the expression of miRNA-135 in hormone-dependent prostate cancer was higher than that in hormone-independent prostate cancer(P<0.05); miRNA-135 could significantly inhibit the growth of DU145 and PC3 cells(P<0.05), especially after 48 h and 72 h. After miRNA-135 transfection, the expression level of STAT6 in prostate cancer cell line DU145 and PC3 was significantly down-regulated compared with NC-transfected cell lines. Conclusion miRNA-135 can inhibit the proliferation of hormone-independent prostatic cell DU145 and PC3, which is expected to be a potential new therapeutic target for prostate cancer.更多还原