[Objective] To clone and sequence the alkane hydroxylase gene alkB of a petroleum hydrocarbon degrading bacteria TY22,then to clone alkB into express vector for expressing and identifying its function.[Method]First,use PCR amplification to get partial sequence of 1236 bp alkB gene,then obtain the complete CDS sequence by chromosome walking techniques.Second,clone alkB gene into pET21b(+) vector,and transform the recombinant plasmid into E.coli BL21(DE3) for expressing.Third,clone alkB gene into pCom8 vector...
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