[Objective] To establish a highly efficient and stable technology of hardening seedling for tissue culture seedling of Eustoma grandiflorum.[Method] Taking tissue culture seedlings which were obtained by stem with young bud of seedlings of E.grandiflorum as experimental materials,the survival rate of the tissue culture seedling,growth development of the roots and the stem leaves were studied under the condition of different cultivation substrate,temperature,root length of seedling.[Result]The hardening seed...