【摘要】 为了解慈竹WRKY生物学功能以及通过基因工程手段改良竹类植物。利用已获得慈竹转录组数据,克隆获得2条WRKY转录因子,分别命名为BeWRKY1（Gen Bank:KJ462124）和Be WRKY2（Gen Bank:KJ462125）,两者分别编码189和295个氨基酸残基。序列分析表明,两者编码的蛋白均含有一个完整的WRKY superfamily的保守结构域。BeWRKY1属于Ⅱc亚家族;Be WRKY2则与Ta WRKY16和AtWRKY39等聚为一枝,属于Ⅱd亚家族。定量PCR分析表明,Be WRKY1和Be WRKY2分别在茎和完全展开叶中具有较高表达量。200mmol·L^–1NaCl和20%PEG 6000胁迫处理激活了Be WRKY1表达,却减少了BeWRKY2的转录积累。结果表明BeWRKY1可能短时间内迅速参与逆境响应;Be WRKY2对干旱和ABA胁迫伤害更为敏感,而可能不参与高盐类胁迫保护反应。更多还原

【Abstract】 In order to explore the biological function of WRKY in Bambusa emeiensis then modify bamboo plants through genetic engineering.Two WRKY genes were cloned based on RNA transcriptome data of Bambusa emeiensis,which were named as BeWRKY1（Gen Bank:KJ462124） and BeWRKY2（Gen Bank:KJ462125）,and encoded 189 and 295 amino acids,respectively.Blastp analysis indicated that both of them contained a WRKY conserved domain.BeWRKY1 belonged to Ⅱ c subfamily,whereas Be WRKY2,aligned with Ta WRKY16 and At WRKY39,belonged to Ⅱ d subfamily.Real-time PCR analysis showed that Be WRKY1 exhibited higher expression in stems,while the expression level of BeWRKY2 was higher in the full expanded leaves.200 mmol·L^-1NaCl and 20% PEG 6000 stresses triggered Be WRKY1 expression,but decreased the transcript accumulation of Be WRKY2.The results showed that Be WRKY1 may response to stress in short time.Be WRKY2 was more sensitive to drought andABA stresses,but did not participate in the protection of high salt stress response.更多还原