【摘要】 目的观察滋肾活血方对冠状动脉粥样硬化性心脏病(冠心病)肾虚血瘀证大鼠缺血心肌病理的影响。方法选择60只大鼠按随机数字表分为空白组、模型组、高剂量组、中剂量组、低剂量组,每组12只。以"左冠状动脉前降支结扎加注射氢化可的松法"制备大鼠心肌缺血肾虚血瘀证模型。高、中、低剂量组分别灌胃滋肾活血方药液21.6、10.8、5.4g/(kg·d),空白组和模型组灌胃等量纯净水,连续灌胃7日。取大鼠心脏,采用光镜进行病理形态学观察。结果模型组大鼠心肌纤维肿胀变形,肌纤维间距增宽,组织疏松水肿,心肌间质血管扩张,较多炎细胞浸润;心肌细胞明显肿胀变性坏死,核溶解,部分核消失;大量胶原纤维组织增生。心内膜血管肿胀变性及少量炎细胞浸润;心外膜组织结构破坏并增生,血管肿胀变性坏死,较多炎细胞浸润和胶原沉积。滋肾活血方各剂量组大鼠心肌病理形态学损伤减轻,随着药物浓度的升高,心肌组织损伤程度逐渐减轻,受损范围逐渐局限。结论滋肾活血方可以减轻肾虚血瘀证模型大鼠缺血心肌病理形态学损伤,调节心肌功能,达到改善冠心病心肌缺血肾虚血瘀证的功效。更多还原

【Abstract】 Objective To observe the effect of Zishen Huoxue Recipe(ZHR)on pathomorphology in coronary heart disease(CHD)rats with Shen deficiency blood stasis syndrome(SDBSS).Methods Totally 60 healthy Wistar rats were divided into the blank control group,the model group,high,middle,and low dose ZHR groups according to random digit table,12 in each group.Myocardial ischemia SDBSS rat model was prepared by ligating the left anterior descending coronary artery and injecting hydrocortisone.ZHR physic liquor was administered to rats in high,middle,and low dose ZHR groups at the daily dose of 21.6,10.8,5.4 g/kg by gastrogavage for 7successive days,equal volume of pure water was administered to rats in the blank control group and the model group by gastrogavage for 7 successive days.Rat heart was collected for pathomorphological observation under light microscope.Results In the model group the heart muscle fiber was swollen and deformed with widened space,loose and dropsy tissues.Blood vessels in myocardial mesenchymal were dilated,infiltrated with more inflammatory cells.Myocardial cells were markedly swollen,degenerated,or necrotic,with caryolysis or disappearance of partial nuclear.A large amount of collagen fibrous tissue became hyperplasia.Endocardial blood vessels were swollen and degenerated with infiltration of few inflammatory cells.Epicardium tissue and structure were destroyed and got hyperplasia.Swollen,degenerated,or necrotic vessels could be seen,with infiltration of more inflammatory cells and collagen deposition.Pathomorphological injuries were alleviated in each ZHR group.The higher ZHR concentration,the milder the injury degree of myocardial tissue,the more limited range of damage.Conclusion ZHR could attenuate pathomorphological injuries of myocardial ischemia rats with SDBSS and regulate myocardial function,thus improving myocardial ischemia in CHD rats with SDBSS.更多还原