The effects of different dosages of PEG6000 on the absorbance( OD680),biomass,growth rate,soluble protein content,lipase activity and total lipid content of chlorella( Chlorella sp. TLD6B) were studied by disposable culture method. The results showed that different dosages of PEG6000 had inhibitory influences on the absorbance( OD680),biomass,growth rate and soluble protein content. With culture time prolonging,the lipase activity showed an increasing,decreasing then increasing trend at the same dosage of P...