【摘要】 目的探讨PCOS患者卵泡液中骨形成蛋白BMP6、BMP15、GDF9的异常改变与颗粒细胞凋亡的相关性及对IVF结局的影响。方法将138例研究对象分为PCOS组与正常排卵组,对两组患者的临床及IVF批标进行对比分析,同时对两组患者的卵泡液行BMP6、BMP15、GDF9检测,且检测了两组颗粒细胞的Bcl-2及Caspase-3的表达情况,进而就卵液中的BMP6、BMP15、GDF9水平与颗粒细胞内的Bcl-2及Caspase-3表达量之间进行相关性分析。结果 PCOS组与对照组相比,患者的年龄、初潮年龄、Gn用量、获卵数、MⅡ卵率、受精率、优质胚胎率及临床妊娠率均无显著差别(P>0.05),而BMI指数及可移植胚胎率均小于对照组,且差异具有统计学意义(P<0.05)。PCOS组与对照组相比,患者卵丘颗粒细胞内Bcl-2 m RNA及蛋白水平显著降低,而Caspase-3m RNA及蛋白水平升高,且差异具有统计学意义(P<0.05)。卵泡液中卵源性蛋白BMP6、BMP15与颗粒细胞内凋亡调控因子Bcl-2有呈正相关,与Caspase-3呈负相关性(P<0.05)。GDF9与Caspase-3呈负相关性(P<0.05),与Bcl-2有一定相关性,但无统计学意义。结论 PCOS患者不仅卵泡液中存在BMP6、BMP15、GDF9的异常体变,且存在颗粒细胞内凋亡调控因子的异常改变,同时二者之间存在一定的相关性。更多还原

【Abstract】 Objective:To investigate the potential effect of BMP6,BMP15 and GDF9 abnormal changes in follicular fluid on IVF outcome through the granulosa cell apoptosis. Methods:A total of 70 PCOS patients(PCOS group)and 68 female patients with normal ovarian function(control group)we collected. The general information,ages,retrieved oocyte number,BMI,Gn dosage,menarche age,fertilization rate,portable embryo rate,high-quality embryo rate,clinical pregnancy rate were recorded. Real-time PCR was used to detect the m RNA levels of Bcl-2 and Caspase-3 in the granulosa cell of all subjects. Western blotting was used to detect the expression of Bcl-2 and Caspase-3 in the granulosa cell and BMP6,BMP15 and GDF9 in the follicular fluid of all subjects. Results:Compared with control group,the BMI and portable embryo rate was significantly decreased(P<0.05),the other general information have no significant differences(P>0.05). The BMP6,BMP15 and GDF9 expression were significantly decreased in PCOS group compared with the control group(P<0.05). The m RNA and protein levels of Bcl-2 were significantly increased and the Caspase-3 was significantly decreased in PCOS group compared with the control group(P<0.05). The BMP6 and BMP15 were positively correlated with Bcl-2(P<0.05)and negatively correlated with Caspase-3(P<0.05). GDF9 was has negative correlation with Caspase-3(P<0.05)and has certain correlation with Bcl-2,but without statistical significance(P>0.05). Conclusions:From these data above we supposed that the low expression of BMP6,BMP15 and GDF9 may affect oocyte quality and embryo development potential and be correlated with the granulosa cell apoptosis.更多还原