【摘要】 目的研究参附注射液（SF）对前列腺癌PC-3细胞免疫逃逸的影响及可能机制。方法实验设立空白对照组（包括PC-3细胞组、淋巴细胞Jurkat细胞组、PC-3-Jurkat共培养组）,参附注射液低、中、高(5,10,20μL·m L^-1)剂量组。用MTT法检测PC-3细胞活性,Hoechst 33258检测PC-3细胞凋亡,Annexin V/PI染色流式细胞术检测Jurkat细胞凋亡,同时用流式细胞术检测PC-3细胞表面CD95表达。结果分别与对照组比较,作用24 h后,SF中、高剂量组PC-3细胞凋亡增多（P<0.05）,SF低、中、高剂量组Jurkat细胞凋亡减少,同时SF低、中、高剂量组PC-3细胞表面CD95阳性表达率增高。结论 SF对PC-3-Jurkat共培养体系中淋巴细胞凋亡有抑制作用,增强PC-3细胞表面CD95表达,SF可能通过此途径阻滞前列腺癌细胞的免疫逃逸,从而发挥抗肿瘤作用。更多还原

【Abstract】 Objective To study the influence of Shenfu injection（SF）on immune escape of prostate cancer PC-3 cell,and to investigate the potential mechanism. Methods Blank control group and SF 5,10,20 μL·m L^-1 groups were set up in this study,prostate cancer PC-3 cells were cultured together with Jurkat cells. PC-3 cell proliferation was analyzed by MTT assay,PC-3 cell apoptosis was measured after Hoechest33258 staining,Jurkat cell apoptosis was measured by flow cytometry after Annexin V/PI staining,and CD95 expression at the PC-3 cell surface was measured by flow cytometry. Results Compared with the blank control group,the number of apoptotic PC-3 cells in SF 10,20μL·m L^-1 groups was increased and that of Jurkat cells in SF 5,10,20 μL·m L^-1 groups was decreased after treatment for 24 h（P < 0.05）. Moreover,CD95 expression at PC-3 cell surface was increased in SF 5,10,20 μL·m L^-1 groups.Conclusion SF can inhibit the apoptosis of lymphocytes co-cultured with PC-3-Jurkat cells, increase CD95 expression at PC-3 cell surface,which may contribute to the blocking of the immune escape of prostate cancer cells and to the antitumor effect.更多还原