【摘要】 目的:在1型单纯疱疹病毒侵袭感染BV2细胞后进行分子检测,分析柯里拉京对感染后BV2细胞后TLR9、My D88mRNA在BV2细胞的表达情况。方法:建立细胞模型(BV2细胞系)柯里拉京的用药浓度及干预时间。而且经过MTT法进行核实,TCID50病毒滴度计算,需要采用Reed-Muench法。细胞随机分为6组:HSV-1+柯里拉京组、HSV-1+无菌PBS组、CPG-ODN+柯里拉京组、CPG-ODG+无菌PBS组、无菌PBS+柯里拉京组、无菌PBS+无菌PBS组(空白对照组)。荧光定量聚合酶链反应(PCR)对上述每组TLR9mRNA以及My D88mRNA表达进行检测。结果:HSV-1+柯里拉京组与HSV-1+PBS组相比TLR9、My D88mRNA的表达降低有统计学差异(P<0.05);CPG-ODN+柯里拉京组与CPG-ODN+PBS组相比TLR9、My D88mRNA的表达降低有统计学差异(P<0.05)。结论:在BV2细胞接受感染HSV-1后,柯里拉京降低TLR9/My D88mRNA的表达,以此来上调免疫应答。更多还原

【Abstract】 Objective: By herpes simplex virus( HSV) type 1 infecting BV2 cells,to observe the expression of TLR9 and My D88 mRNA. Methods: BV2 cell line of HSV-1 encephalitis was set up. Determine the corilagin concentration and intervention time by MTT,and calculate the TCID50 of virus titer by the Reed-Muench.We randomly divided cells into six groups: HSV-1 + corilagin group,HSV-1 + PBS group,Cp G-ODN +group,corilagin,CPG-ODG + PBS group,PBS + corilagin group,PBS + PBS group( the control group).The expression of TLR9 / My D88 mRNA was measured by FQ-PCR and TLR9. Results: Compared with HSV-1 + PBS group,HSV-1 + Corilagin decreased the TLR9 and My D88 mRNA expressions( P < 0. 05);compared with Cp G-ODN + PBS group,Cp G-ODN + Corilagin decreased the TLR9 and My D88 mRNA expressions( P < 0. 05); Conclusion: TLR9 / My D88 signaling pathway may be key point in immune response in the HSE by the affected TNF-alpha. Corilagin can reduce TLR9 and My D88 mRNA expressions.更多还原