【摘要】 目的分析荆门市2010—2014年流感哨点监测结果,掌握流感病毒的变异、变化及流行规律。方法收集流感样病例监测资料,用逆转录-聚合酶链反应(RT-PCR)法进行流感病毒检测,用狗肾传代细胞法进行流感病毒鉴定。结果 2010-2014年共检测3 111份流感样病例,分离流感病毒367株,阳性率为11.80%;其中季节性H3亚型171株、新甲型H1N1 85株、B型111株;4年间3种流感亚型交替流行,2010年B型和季节性H3亚型为主,2011年新甲型HINI为主,2012年B型和新甲型HINI为主,2013年以新甲型H1N1和季节性H3亚型为主,2014年以季节性H3亚型为主。结论 2010-2014年荆门市流感病毒株的主导型别每年都发生变化,流感流行整体处于相对平稳状态。更多还原

【Abstract】 Objective To explore the sentinel surveillance results of influenza in Jingmen of Hubei during 2010- 2014,obtain the features of influenza virus variation,development and epidemic. Methods The surveillance data of influenza was collected,and the influenza virus test was used the real- time polymerase chain reaction( RT- PCR),combining the method of Madin- Darby Canine Kidney cells to identify the virus strain. Results 2010- 2014,a total of 3 111 specimens of influenza virus were collected,and 367 positive( 11. 80%). There were 171 strains of A( H3),85 strains of A( H1NI) and 111 strains of type B. There kinds of influenza subtype were alternately epidemic in 2010- 2014. The dominant epidemic strain of influenza virus was type B and A( H3) in 2010,A( H1N1) subtype in 2011,type B and A( H3) subtype in2012,A( H3) subtype and A( H1N1) in 2013 and A( H3) subtype in 2014. Conclusion During 2010-2014,the dominate strains of influenza virus in Jingmen were alternated in different year and influenza epidemic performed a stable trend.更多还原