【摘要】 目的:测定黄蜀葵花总黄酮中金丝桃苷的平衡溶解度和表观油水分配系数。方法:采用HPLC法测定金丝桃苷在水、不同有机溶剂和不同pH缓冲液中的平衡溶解度,用摇瓶法测定金丝桃苷在正辛醇-水/缓冲液中的表观油水分配系数。结果:37℃下金丝桃苷在水中的平衡溶解度为231.58 mg·L^-1,表观油水分配系数为0.907（log Papp=-0.04）;常用有机溶剂甲醇对金丝桃苷的溶解性较好,为6 579.21 mg·L^-1;在不同pH缓冲溶液中,溶解度随着pH的升高逐渐升高,油水分配系数随着pH的升高逐渐降低。结论:金丝桃苷的水溶性差,pH对其溶解度和表观油水分配系数均有较大影响。更多还原

【Abstract】 Objective:To determine the equilibrium solubility and the apparent oil/water partition coefficient（Papp） of hyperin in total flavonoids from Abelmoschus Manihot.Methods:An HPLC method was used to detect the concentration of hyperin in water,different organic solvents and different pH buffer solutions.The partition coefficients of hyperin in n-octanol-water/buffer solutions were determined by a shaking flask method.Results:The equilibrium solubility of hyperin was 231.58 mg·L^-1 in water at 37℃ and Papp was 0.907（log Papp=-0.04）.The higher equilibrium solubility of hyperin was 6 579.21 mg·L^-1 in methanol; in different pH buffer solutions,its solubility gradually increased with the increase of pH,and Papp gradually decreased with the increase of pH.Conclusion:The studies showed that hyperin has poor solubility in water,and its solubility and Papp are both highly related with pH.更多还原