【摘要】 目的:建立了抗毒合剂的指纹图谱分析方法。方法:采用高效液相色谱法,色谱条件:Agilent C₁₈色谱柱（150 mm×4.6 mm,5μm）,流动相:0.1%磷酸水-乙腈,梯度洗脱,流速:1.0 ml·min^-1,柱温:25℃,检测波长:290 nm。结果:在选定的色谱条件下确定了17个共有峰构成10批抗毒合剂的指纹特征指纹图谱,各批样品的相似度均大于0.9。结论:该法精密度高,重复性好,可为抗毒合剂的指纹图谱评价提供依据。更多还原

【Abstract】 Objective: To establish a fingerprint analysis method for Kangdu mixture. Methods: An HPLC method was used with the following chromatographic conditions: an Agilent C₁₈ chromatographic column（ 150 mm × 4. 6 mm,5 μm） was applied,the mobile phase was 0. 1% phosphoric acid water and acetonitrile with gradient elution at a flow rate of 1. 0 ml·min^-1,the column temperature was 25 ℃,and the detection wavelength was 290 nm. Results: Under the selected chromatographic conditions,17 common peaks were identified to construct the fingerprint for 10 batches of Kangdu mixture,and the similarity of the samples was above 0. 9. Conclusion:The established method is with high precision and good reproducibility,which can be applied in the fingerprint evaluation of Kangdu mixture.更多还原