【摘要】 目的:制备痤疮消炎洗剂,建立测定该制剂高效液相色谱指纹图谱的方法,用于控制该制剂的质量。方法:采用HPLC梯度洗脱法测定痤疮消炎洗剂的指纹图谱,色谱柱为Waters XTerra MS C18柱（250 mm×4.6 mm,5μm）;乙腈为流动相A,0.2%磷酸溶液为流动相B,流速:1.0 ml·min^-1,检测波长:277 nm,柱温:30℃。结果:标示出痤疮消炎洗剂中16个共有峰,并确证3个已知峰为黄芩苷、蒙花苷和大黄酸。结论:该法建立的痤疮消炎洗剂的H PLC指纹图谱重复性好,色谱峰信息量大,为全面控制该制剂的质量提供了可靠的方法。更多还原

【Abstract】 Objective: To prepare Cuochuang Xiaoyan lotion and establish HPLC fingerprint chromatogram for the quality control.Methods: The separation was performed on a Waters XTerra MS C18column（ 250 mm × 4. 6 mm,5 μm）. The mobile phase consisted of acetonitrile-0. 2% phosphoric acid with gradient elution at a flow rate of 1. 0 ml·min^-1,the eluent was monitored by a UV detector at 277 nm,and the column temperature was at 30℃. Results: There were sixteen common peaks for the sample,and among them,three ones were identified as baicalin,linarin and rhein,respectively. Conclusion: The repeatability and information of chromatogram peaks of the method are satisfied,which can provide credible quality control method for Cuochuang Xiaoyan lotion.更多还原