【摘要】 目的使孕中期雌鼠暴露于高雄激素状态,观察宫内高雄激素环境是否影响子代的行为,并探讨产生焦虑行为的可能机制及多囊卵巢综合征与情志的关系。方法 Wistar雌性大鼠,分别在孕中期颈背部皮下注射T、T+Flu、T+Tam,连续3 d,所生子代雌鼠及雄鼠长至成年作为实验对象,观察基本生长情况、行为表现,检测中枢神经系统下丘脑、海马和杏仁核中关键基因的表达情况。结果 1)T组杏仁核ARm RNA的表达低于对照组。2)与对照组相比,T组子代的海马组织中ERαm RNA的表达下降。3)在T+Flu组中,下丘脑和海马中GPER m RNA的表达比T组下降。与T组相比,GPER m RNA在T+Flu和T+Tam组杏仁核中的表达显著增加。结论通过试验发现PNA大鼠焦虑行为表现更为明显,其异常的表现是通过雄激素途径发挥作用。说明子代鼠在宫内对母鼠孕期持续的高雄激素刺激产生应激,雄激素途径对子代鼠中枢神经系统产生作用,从而影响其出生后的焦虑行为。更多还原

【Abstract】 Objective: To observe whether the androgen excess in utero environment affected the behavior of the offspring,and to explore the possible mechanism of anxiety and the relationship between polycystic ovary syndrome and emotion after the female mice were exposed to the androgen excess in the second trimester of pregnancy. Methods: T,T+Flu and T+Tam were used in the second trimester of pregnancy on Wistar rats for three days.The basic conditions,behaviours and the expression of key genes in the hypothalamus,hippocampus and amygdala of the central nervous system of the mice offspring were observed. Results: 1)The expression of ARm RNA in T group was lower than that in the control group. 2)Compared with the control group,the expression of ERαm RNA in the mice offspring in T group decreased. 3)In T +Flu group,the expression of GPER m RNA in hypothalamus and hippocampus decreased compared with that in T group. Compared with T group,the expression of GPER m RNA in T+Flu and T+Tam groups increased significantly. Conclusion: The anxiety behavior of PNA rats is more obvious,and the abnormal expression of the rats is achieved by the androgen pathway. It shows that the mice offspring is involved in stress responses to excessive androgen environment during the pregnancy of the female rats.It affects the central nervous system of the mice offspring by the androgen pathway thus producing the anxiety behaviours.更多还原