【摘要】 目的为明确复心汤治疗心衰的作用靶点,以心肌细胞信号转导分子GATA4、PKA、ERK作为观察对象,观察复心汤对GATA4、PKA、ERK表达的影响。方法健康成年的雄性Wistar大鼠75只,随机分为复心汤低剂量组、复心汤中剂量组、复心汤高剂量组、缬沙坦组、生理盐水组,制备含药血清,原代乳鼠心肌细胞的培养,加入相应含药血清干预24 h,Western blot法测各组乳鼠心肌细胞中GATA4、PKA、ERK表达。结果复心汤3组及缬沙坦组乳鼠心肌细胞中GATA4、PKA、ERK表达量明显低于生理盐水组(P<0.01),复心汤中剂量组抑制GATA4、PKA、ERK表达方面较复心汤低、高剂量组及缬沙坦组更明显(P<0.01)。结论复心汤通过抑制心肌细胞GATA4、PKA、ERK表达,抑制心室肥厚,改善心衰。更多还原

【Abstract】 Objective: In myocardial cell signal transduction molecules GATA4,PKA,ERK as research object,This research through observation Fu Xin Decoction for the effect of GATA4、PKA、ERK expression,For the clear Fu Xin Decoction′ s targets for the treatment of heart failure. Methods: 75 healthy adult male Wistar rats were randomly divided into low dose group,median dose group and high dose group of Fu Xin Decoction,valsartan group,normal saline group. Medicated serum were prepared. The cultivation of the myocardial cells and processing,join the drug-containing serum levels of processing for 24 hours. The expression of myocardial cells GATA4,PKA,ERK were detected with the method of Western blot. Results: Compared with normal saline group,low dose group,median dose group and high dose group of Fu Xin Decoction,valsartan group,GATA4,PKA,ERK in rat myocardial cells,expression quantity to decrease,with statistically significant difference(P < 0.01);Compared with median dose group after low dose group,high dose group of Fu Xin Decoction,valsartan GATA4,PKA,ERK in rat myocardial cells,expression quantity increased obviously had a statistically significant difference(P < 0.01). Conclusion: Fu Xin Decoction can by adjusting the rat myocardial cells GATA4、PKA、ERK to achieve the goal of treatment for heart failure.更多还原