【摘要】 目的观察人参皂苷对波动性高糖大鼠氧化应激及血管病变的影响。方法将48只雄性SD大鼠随机分成正常对照组8例和糖尿病模型组40例。高脂饲料喂养糖尿病模型组大鼠2周后用小剂量链脲佐菌素(STZ)诱导建立糖尿病大鼠模型,之后随机分为稳定高糖组(n=8)和波动性高糖组(n=32),波动性高糖组错时注射葡萄糖、胰岛素制备血糖波动大鼠模型。2周后,将波动性高糖组随机分为中药低剂量组[人参皂苷14 mg/(kg·d)],中药中剂量组[人参皂苷28 mg/(kg·d)],中药高剂量组[人参皂苷56 mg/(kg·d)]及波动性高糖模型组。实验结束后测定大鼠血清一氧化氮(NO)、一氧化氮合酶(NOS)、谷胱甘肽过氧化物酶(GSH-Px)浓度并取肾脏及主动脉组织并做常规病理。结果 1)与正常对照组相比,稳定高糖组及波动性高糖模型组血清NO、NOS、GSH-Px浓度均明显增加(P<0.05);与稳定高糖组比较,波动性高糖模型组NO、NOS、GSH-Px浓度明显增加(P<0.05);与波动性高糖模型相比,中药各剂量组NO、NOS浓度均有明显下降,GSH-Px浓度均有明显上升(P<0.05);与中药低剂量组相比,中药中、高剂量组有降低NO、NOS浓度,升高GSH-Px浓度的趋势(P<0.05);与中药中剂量组相比,中药高剂量组有降低NO、NOS浓度,升高GSH-Px浓度的趋势(P<0.05)。2)与正常对照组相比,稳定高糖组及波动性高糖模型组主动脉内皮细胞出现一定程度的紊乱与损伤,肾小球体积不同程度的增大变形。结论人参皂苷可降低波动性高血糖大鼠NO、NOS水平,升高GSH-Px水平,减轻血管病变情况,因此,人参皂苷可能降低波动性高血糖大鼠机体的氧化应激水平,提高抗氧化的能力,对波动性高血糖所导致的血管病变有一定的保护作用。更多还原

【Abstract】 Objective: To observe the effects of ginsenoside on oxidative stress level and vascular disease in high blood glucose fluctuation state of diabetic rats. Methods: Forty eight male Sprague Dawley(SD) rats were randomly divided into the normal group(group N,n = 8) and diabetic model group(n = 40). The diabetic rat model was established with injection of a small dose of streptozotoein(STZ) after feeding them with high fat diet for 2weeks,and then were randomly divided into the sustained high glucose diabetic rat model group(group S,n = 8)and the high blood glucose fluctuation rat model group(group F,n = 32). The model of high blood glucose fluctuation rat were developed with alternative intraperitoneal injection of insulin and glucose. Successfully-established model rats were randomly divided into four groups: low [14 mg/(kg·d),group FL],middle[28 mg/(kg·d),group FM],high [56 mg/(kg·d),group FH] dosage of ginseng treatment group and control group(group FO). After 8weeks,NO,NOS and GSH-Px were measured and vascular disease of aorta and kidney were observed. Results:1)Compared with group N,levels of NO,NOS and GSH-Px in S and FO groups increased significantly(P < 0.05);compared with group S,levels of NO,NOS and GSH-Px in group FO increased significantly(P < 0.05);compared with group FO,levels of NO and NOS in ginseng treatment groups decreased significantly(P < 0.05);level of GSH-Px increased significantly(P < 0.05);compared with group FL,levels of NO and NOS in FM and FH groups decreased significantly(P < 0.05);levels of GSH-Px increased significantly(P < 0.05);compared with group FM,levels of NO,NOS in FH group were decreased significantly(P < 0.05);level of GSH-PX was increased significantly(P < 0.05). 2)Compared with group N,aortic endothelia cells were disordered and glomenrulus were enlarged and deformed,compared with group FO,morphology changes were improved in ginseng treatment groups.Conclusion: Certain anti-oxidative capability is shown to restrain oxidative stress in ginsenoside,which can play a critical role in treating vascular disease caused by high blood-glucose fluctuation.更多还原