【摘要】 目的:探讨stathmin基因表达水平与紫杉醇联合铂类疗效在卵巢癌中相关性研究及其临床意义。方法:应用Quanti-Gene Assay技术检测88例III~IV上皮性卵巢肿癌患者肿瘤组织中stathmin基因的表达水平,对照分析其与患者年龄、新辅助化疗,残瘤灶,药物敏感度、总生存期/生存率、stathmin蛋白表达的关系。结果:患者年龄越大,肿瘤组织中stathmin基因表达水平越高,但P>0.05,无统计学意义;新辅助化疗后,患者stathmin基因表达量增加,且有显著性差异,P<0.01;术后有残瘤灶患者的stathmin基因表达量较高,而没有残瘤的基因表达量较低,P<0.05,有统计学意义;药物敏感度较低患者的stathmin基因表达量较大,相反,敏感度较大的表达量较小,但通过单因素方差分析发现差异没有达到显著的程度;生存期越长,患者的stathmin基因表达量越低,两者呈负相关性,且有显著意义;OS曲线结果显示stathmin低表达组的生存期明高于stathmin高表达组;stathmin蛋白质强度越大,其RNA表达量就越高,其RNA表达与蛋白质的表达一致,有显著性差异。结论:肿瘤组织中stathmin基因表达水平可能与卵巢癌患者紫杉醇联合铂类耐药及预后密切相关。更多还原

【Abstract】 Objective: To investigate the expressions of stathmin and paclitaxel combined with platinum chemotherapy in ovarian cancer and its clinical significance in correlation study. Methods: The expression of stathmin gene in 88 cases of III and IV epithelial ovarian tumor patients were detected by Quanti-Gene assay technique. Comparatively analyze the relationship with age,neoadjuvant chemotherapy,residual tumor,drug sensitivity and overall survival and survival rate and stathmin. Results: The larger the patients ages were,the higher the stathmin gene expression in tumor tissue was,but there was no statistically significant,P > 0. 05. After neoadjuvant chemotherapy,patients’ stathmin gene expression increased and there was a significant difference,P < 0. 01. The stathmin gene had high expression of residual tumor and low of those with no residual gene expression. There was statistical significance,P < 0. 05. The patients who had lower drug sensitivity had high expression of stathmin gene. On the contrary,those who had sensitive expression had smaller expression. But by single factor variance analysis,it showed that the differences did not reach the significant level. The longer the survival period was,the expression lower of stathmin gene in patients and there was a negative correlation between the two. The OS curve showed stathmin low expression group’s survival was significantly higher than that of stathmin high expression group. The more stronger the stathmin protein strength was,the higher expression level of RNA and the expression of RNA. There were significant differences. Conclusion: The level of stathmin gene expression in tumor tissue may be closely related to the combination of paclitaxel and platinum resistance in ovarian cancer patients.更多还原