【摘要】 目的:分析银杏叶提取物Egb761对迟发性运动障碍(TD)模型大鼠行为学和血清脑源性营养因子(BDNF)和总抗氧化能力(TAC)水平的影响,探索TD可能的防治机制。方法:将32只雄性Sprague-Daw ley(SD)大鼠随机分为对照组[腹腔注射生理盐水(NS)2 m L/kg+第5周始灌胃5 m L/kg NS]、TD组[腹腔注射氟哌啶醇(Hal)2 mg/kg+第5周始灌胃5 m L/kg NS]、Egb761预防组(PEgb761组)(腹腔注射Hal 2 mg/kg同时灌胃5 m L/kg Egb761溶液)、Egb761治疗组(T-Egb761组)(腹腔注射Hal 2 mg/kg+第5周始灌胃5 m L/kg Egb761溶液)。每组8只,共观察10周,每周第7天评定大鼠口周不自主运动(VCM)的严重程度。第10周末采用酶联免疫吸附法(ELISA)检测血清BDNF浓度,采用分光光度法检测血清TAC水平。结果:与对照组相比,第3周末TD和T-Egb761组VCM评分显著增加(P<0.05),第5周末达峰值;第6周后T-Egb761组VCM评分逐步下降;第10周末T-Egb761组VCM评分(4.1±2.0)显著低于TD组(27.9±5.8)(P<0.001),与对照组(3.5±1.9)无差异(P>0.05);P-Egb761组与对照组10个评估点VCM评分无差异;第10周末,TD组血清BDNF水平[(6.9±1.0)pg/m L]低于对照组[(8.6±2.5)pg/m L]、T-Egb761组[(8.9±1.5)pg/m L]和P-Egb761组[(9.6±1.4)pg/m L];TD组TAC水平[(11.9±3.2)U/m L]低于对照组[(18.2±5.5)U/m L]和T-Egb761组[(19.4±4.4)U/m L](P<0.05),与P-Egb761组差异无统计学意义(P>0.05)。结论:Egb761可预防和显著缓解TD模型大鼠VCM症状,神经元的保护因素和自由基代谢异常可能在TD发生、发展过程中作用关键。更多还原

【Abstract】 Objective:To investigate the efficacy of medication and prevention of ginkgo biloba extract Egb761 on vacuous chewing movements(VCMs) of haloperidol-induced tardive dyskinesia(TD) rats and serum levels of brain-derived neurotrophic factor(BDNF) and total antioxidant capacity(TAC),and to explore the possible mechanisms.Methods:Thirty-two male Sprague-Dawley(SD) rats were randomly divided into the control,TD,P-Egb761,T-Egb761 group(n = 8),processed with normal saline(NS),haloperidol(Hal) + NS,Hal +Egb761(medicated at the baseline),Hal + Egb761(medicated at the 5th week) with 8 rats in each group,the test duration was 10 weeks.VCM was evaluated at each weekend.Venous blood was collected at the end of 10 th week,and the serum levels of BDNF and TAC were assayed.Results:At the 10 th weekend,the VCM score of T-Egb761group(4.1 ± 2.0) were significantly lower than those of the TD group(27.9 ± 5.8)(P < 0.001).At the 10 th weekend,serum BDNF levels[(6.9 ± 1.0) pg/mL]of TD group were significantly lower than those of the controls[(8.6 ±2.5) pg/mL],P-Egb761[(9.6±1.4) pg/mL]and T-Egb761[(8.9 ±1.5) pg/mL]groups.The TAC levels were lower in the TD group[(11.9 ±3.2) U/mL]than those in the control group[(18.2 ±5.5) U/mL]and T-Egb761 group[(19.4 ±4.4) U/mL](P<0.05),and had no significant difference with those in the PEgb761 group(P>0.05).Conclusion:It suggests that Egb761 might alleviate and prevent the symptoms of abnormal involuntary movement in TD model rats,and the protective factors of neurons and free radicals metabolism might play key role in etiology of TD.更多还原