Short-chain fatty acids(SCFAs) play a key role in altering carbohydrate and lipid metabolism, influence endocrine pancreas activity, and as a precursor of ruminant milk fat. However, the effect and detailed mechanisms by which SCFAs mediate bovine growth hormone(GH) and prolactin(PRL) gene transcription remain unclear. In this study, we detected the effects of SCFAs(acetate, propionate, and butyrate) on the activity of the cA MP/PKA/CREB signaling pathway, GH, PRL,and Pit-1 gene transcription in dairy cow a...