【摘要】 目的观察骨髓间充质干细胞对慢性环孢素肾病(CCN)的治疗作用。方法实验大鼠分成3组:正常对照组(C组)、模型组(H组)、骨髓间充质干细胞(BMSC)治疗组(B组);动态监测肾脏功能[血清肌酐(Scr)、血清尿素氮(BUN)]及大鼠生长情况变化,28 d处死大鼠并切取肾脏进行组织形态学检查和肾间质纤维化(RIF)评估,RIF比较采用方差分析和大鼠体重、Scr、尿素水平采用两因素重复测量方差分析比较各组差异。结果与C组比较,H组和治疗组的Scr[(30.07±1.25)μmol/l vs(69.40±1.41)μmol/l,(51.04±1.38)μmol/l,P=0.00]、BUN水平[(9.05±0.57)mmol/l vs(16.80±0.51)mmol/l,(13.12±0.57)mmol/l,P=0.00]均显著升高,体重显著下降[(329.68±5.71)g vs(199.98±5.59)g,(226.22±5.91)g,P=0.00],差异具有统计学意义。与H组比较,B组对大鼠的Scr[(69.40±1.41)μmol/l vs(51.04±1.38)μmol/l,P=0.00]、BUN水平有明显下降[(16.80±0.51)mmol/l vs(13.12±0.57)mmol/l,P=0.00],体重增加[(199.98±5.59)g vs(226.22±5.91)g,P=0.00],差异具有统计学意义。肾组织形态上,与C组比较,模型组与治疗组都出现了明显的肾脏损害,肾间质纤维化(RIF)评分均显著升高(0.253±0.030 vs 2.662±0.025,2.256±0.027,P=0.00),差异具有统计学意义;与H组比较,B组RIF评分显著下降(2.662±0.025 vs 2.256±0.027,P=0.00),差异具有统计学意义。结论 BMSC对CCN大鼠有改善作用,可以显著减少大鼠Scr、BUN水平,改善大鼠体重情况。更多还原

【Abstract】 Objectives To observe therapeutic effect on chronic cyclosporine nephropathy by bone mesenchymal stem cell(BMSC) technology.Methods Experimental rats were divided into 3 groups:Group C:normal group(control);Group H:chronic cyclosporine nephropathy group;Group B:mesenchymal stem cells therapy group;Dynamically monitor renal function(serum creatinine and urea nitrogen) and rat growth change,take kidney tissue for tectology evaluation on 28 d,meanwhile,test the expression levels of TGF-β1.Results Compared with group C,group H and group B have serum creatinine[(30.07±1.25)μmol/l vs(69.40±1.41)μmol/l,(51.04±1.38) μmol/l,P=0.00]and urea nitrogen [(9.05±0.57)mmol/l vs(16.80±0.51)mmol/l,(13.12±0.57)mmol/l,P=0.00]significantly raised and body weight[(329.68±5.71)g vs(199.98±5.59)g,(226.22±5.91)g,P=0.00] significantly dropped,with statistically significant difference.Compared with group H,rats in group B are improved on body weight and renal function,with serum creatinine[(69.40±1.41)μmol/l vs(51.04±1.38)μmol/l,P=0.00] and urea nitrogen levels[(16.80±0.51)mmol/l vs(13.12±0.57)mmol/l,P=0.00] significantly dropped and body weight [(199.98±5.59)g vs(226.22±5.91)g,P=0.00]gained.For kidney tectology,group H and group B both show significant kidney damage and different degrees of tubular interstitial fibrosis,with RIF scores significantly increased(0.253±0.030 vs 2.662±0.025,2.256±0.027,P=0.00);and compared with group H,rats in group B have interstitial fibrosis significantly alleviated,with RIF scores dropped significantly(2.662±0.025 vs 2.256±0.027,P=0.00).Conclusion Bone mesenchymal stem cell therapy can improve health of rats with chronic cyclosporine nephropathy.更多还原