This study suggests that the new Y subfamily of pp Gal NAc-Ts plays an important role in protein glycosylation; characterizing their functions will provide new insight into the role of pp Gal NAc-Ts. Disulfide- and terminal alkyne-modified magnetic silica particles(DA-MSPs) were synthesized and used to covalently capture and reductively release azido glycopeptides via click chemistry and dithiothreitol treatment. Using DA-MSPs, an efficient and specific enrichment method for separating azido glycopeptides h...