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缺氮胁迫下含Na~+叶面肥对花生生长的抑制及补氮后的恢复效应

Inhibition of Na~+-containing foliar fertilizer on peanut growth under N deficiency stress and recovery effect by supplemental N spraying

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【作者】 沈浦冯昊罗盛于天一郑永美吴正锋陈殿绪孙学武王才斌郑亚萍

【Author】 SHEN Pu;FENG Hao;LUO Sheng;YU Tian-yi;ZHENG Yong-mei;WU Zheng-feng;CHEN Dian-xu;SUN Xue-wu;WANG Cai-bin;ZHENG Ya-ping;Shandong Peanut Research Institute;

【机构】 山东省花生研究所

【摘要】 【目的】叶面肥中的钠离子(Na+)大多为伴随离子,其对作物的影响通常被忽视。实践中,Na+对作物的作用包括促进和抑制两方面,取决于作物营养生理状况。研究Na+对花生的抑制与缺氮(N)胁迫的关系,探索补施氮素对上述抑制作用的恢复效应,可为花生高效合理施肥提供理论依据。【方法】盆栽试验在山东省花生研究所人工气候室内进行,设喷施肥料中含Na+0、2.0、10.0 mg/pot 3个水平,依次记为T0、T1和T2。测定了花生叶片光合作用、茎叶及根干物质累积、根系形态特性和养分吸收,比较了缺氮胁迫(T0、T1、T2)和后期补施尿素态N(T0-N、T1-N、T2-N)条件下Na+对花生影响的差异,并深入分析花生Na吸收与干物质累积和氮(N)、磷(P)、钾(K)养分吸收的相关关系。【结果】1)缺氮胁迫条件下,叶面喷施Na+显著抑制了花生的光合作用,净光合速率由CO2 11.4μM/(m2·s)下降到CO2 6.77.5μM/(m2·s)。茎叶和根干重T0(6.4和2.4 g/pot)>T1(5.7和1.9 g/pot)>T2(5.3和1.5 g/pot)。与T0相比,根总长度、总表面积和总体积T1处理下降了7.8%10.5%,T2处理显著下降了27.5%31.8%。植株Na与N、K含量呈显著负相关关系(P<0.05),花生Na吸收量每增加1.0 mg/pot,植株总的N、K吸收量分别下降26.6和20.9 mg/pot。2)后期补充喷施氮素条件下,施Na+处理花生茎叶干物重比不施Na+处理增加了20.3%25.8%。补施氮素后,施Na+促进了茎叶对N、P、K的吸收,N的吸收量增加了9.2%19.2%,P增加了20.0%42.3%,K增加了21.4%24.0%。相关性分析表明,花生植株总干物重及P、K含量在补施氮素后与Na含量呈显著正相关关系(P<0.05)。【结论】Na+对花生生长及养分吸收的影响与氮素营养有关。在缺氮胁迫下,Na+对花生的生长为抑制作用,抑制程度随喷施量增加而加重,高浓度Na+显著抑制根茎叶干物质的累积和N、K养分的吸收。补施氮肥提高了花生植株对Na+的适应性,恢复了光合作用及养分吸收能力,消除了Na胁迫的危害作用,产生"N–Na"协同促进效应。

【Abstract】 【Objectives】Sodium(Na+) is often contained as counterion in foliar fertilizers, so its effect on crop growth is least considered during fertilization. Na+ plays both promotion and inhibition roles on crop growth,depending on the plant nutrition and physiology status. The inhibition role of Na+ was studied on peanut exposure to Na+ stress, and the recovery effect of supplemental spraying N to the inhibition was also investigated.【Methods】A pot experiment was conducted inside the growth chamber located in Shandong Peanut Research Institute. Three levels of Na+ were designed in the foliar fertilizer: Na+ 0, 2.0 and 10.0 mg/pot, and recorded as T0,T1 and T2 in turn. The peanut photosynthesis, dry matter accumulation, root morphology, nutrient absorption were investigated in both N deficit stresses(T0, T1, T2) and later urea supplement(T0-N, T1-N, T2-N). The effect of Na absorption on the N, P and K absorption was analyzed. 【Results】1) Without N supplement, the net photosynthetic rates were significantly decreased from 11.4 CO2 μM/(m2·s) to 6.7–7.5 CO2 μM/(m2·s) in Na containing treatments, the dry weights of shoot and root were in order of T0(6.4 and 2.4 g/pot) > T1(5.7 and1.9 g/pot) > T2(5.3 and 1.5 g/pot). Total root length, surface area, and volume in T1 and T2 treatments were decreased by 7.8%–10.5% and 27.5%–31.8% compared with T0. 2) With later supplement of N, Na+ inhibition in photosynthesis was recovered quickly. The shoot dry weights in T1-N and T2-N treatments were increased by 20.3%–25.8% compared with T0-N. The inhibition of Na+ became promotion of N, P, K absorption. The shoot N, P, K absorptions in T1-N and T2-N treatments were respectively increased by 9.2%–19.2%,20.0%–42.3%, 21.4%–24.0% compared with T0-N. The total dry weight of peanut and N, K absorptions were positively correlated with Na+(P < 0.05). 【Conclusions】Under N deficit, Na+ shows inhibition effect on the growth and nutrient absorption of peanut and the effect will become exaggerated with the increased Na+ contents in fertilizers.This inhibition will be remarkably correlated by later supplement of N, and the inhibition role will change to promotion role of Na+. Therefore, application of Na+ containing fertilizer, especially those with high Na+ concentration, should consider the N supply in order to relieve the possibly harmful effect of Na+.

【基金】 山东省农业科学院科技创新重点项目(2014CXZ06-2,2014CXZ11-2);国家自然科学基金项目(41501330,31571617);山东省农业科学院青年科研基金项目(2015YQN05);山东省农业重大应用技术创新课题资助
  • 【文献出处】 植物营养与肥料学报 ,Journal of Plant Nutrition and Fertilizer , 编辑部邮箱 ,2016年06期
  • 【分类号】S565.2
  • 【被引频次】7
  • 【下载频次】176
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