【摘要】 土壤盐渍化是影响农作物生长、发育及产量的非生物胁迫之一,对参与盐胁迫响应的功能基因的研究将有助于揭示植物胁迫响应的分子机制。本实验室前期报道的拟南芥LFR(Leaf and Flower Related)是一个表达广泛、编码细胞核定位蛋白、影响叶和花发育的重要功能基因。本文以lfr功能缺失突变体为实验材料,观察了不同浓度盐处理下的种子萌发、子叶转绿、主根生长及成苗生长表型,结果表明lfr突变体对盐胁迫敏感;定量RT-PCR检测表明在Na Cl处理下,盐胁迫SOS信号通路中的SOS1在lfr中的受诱导程度显著低于野生型,这与lfr突变体盐敏感表型相吻合,可能是导致lfr突变体对盐胁迫敏感的诱因之一。上述结果预示着LFR可能通过调控SOS1基因的转录表达,参与植物对盐胁迫的响应。更多还原

【Abstract】 Salt stress greatly influences plant growth and development.The study of more genes involved in this process will contribute to unravel the mechanism of salt stress.Our previous reports showed that Arabidopsis LFR(Leaf and Flower Related) played an important role in leaf and flower development.Here,we conducted a series of phenotypic analysis,including the seed germination,cotyledon greening and root growth in seeding stage,and the content of chlorophyll at mature plant stage with or without Na Cl treatment.We found that lfr mutants were hypersensitive to salt stress.By means of real-time PCR,we analyzed the expression level of the SOS(salt overly sensitive) genes.No detectable differences were detected for SOS2 and SOS3,however,SOS1 were down-regulated in lfr than the wild type under salt stress condition.In conclusion,these data imply that LFR may involve in salt stress by regulating SOS1 on transcriptional level.更多还原