【摘要】 目的了解北京市朝阳区新确认HIV-1感染者耐药相关位点突变的情况。方法选取2014年朝阳区新确认的HIV-1感染者125例,提取血浆中的病毒RNA,用反转录PCR和套式PCR扩增HIV病毒的pol区,并进行序列测定及耐药分析。结果 92份标本扩增成功,扩增阳性样本亚型分布以CRF01_AE亚型为主,占58.70%,31例(33.70%)为CRF07_BC亚型,6例(6.53%)为B亚型,1例(1.07%)为CRF08_BC亚型;经耐药相关位点分析,27例出现了至少一种耐药相关突变;11例(11.96%)患者携带的耐药相关突变位点可能会引起不同程度的耐药。结论朝阳区新确认HIV-1感染者原发性耐药处于相对较高的水平,与新确认的感染者中以男男性接触人群为主有关,同时提示应当对HIV原发性耐药监测常规化。更多还原

【Abstract】 Objective To examine the prevalence of drug resistance mutations among the newly confirmed HIV-1 infectors in Chaoyang district of Beijing. Methods 125 cases of newly confirmed HIV-1 infection in Chaoyang district in 2014 were selected,and the virus RNA was extracted from plasma. The pol region of HIV virus was amplified by reverse transcription PCR and nested PCR,and the sequence analysis and drug resistance analysis were carried out. Results A total of 92 samples were successfully amplified,and the subtypes distribution of the positive samples was on CRF01_ AE subtype,accounting for58. 70%,31 cases( 33. 70%) were CRF07_BC subtype,6 cases( 6. 53%) were B subtype,1 cases( 1. 07%) was CRF08_BC subtype; drug resistance related mutation analysis showed that 27 cases appeared at least one kind of drug resistance related mutations; in 11 patients( 11. 96%),the drug resistance related mutations may cause different degrees of resistance. Conclusion The prevalence of drug resistance is high relatively in the newly confirmed HIV-1 infectors in Chaoyang district,and maybe MSM is the main cause. Regular surveillance and monitoring of drug resistance HIV should be implemented.更多还原