【摘要】 目的对紫外速率定量法和改良G6PD/6PGD比值法检测女性杂合子G6PD活性进行对比分析和评价,确定出对G6PD缺乏的女性杂合子检出率较高的筛查方法。方法对突变特异性扩增系统(ARMS)法确诊的60例G6PD缺乏女性杂合子及60例G6PD正常病例,分别采用紫外速率定量法和改良G6PD/6PGD比值法进行检测,并对结果进行对比分析。结果紫外速率定量法的灵敏度为65.0%,特异度为96.7%,改良G6PD/6PGD比值法的灵敏度为38.3%,特异度为91.7%,两者对G6PD缺乏的女性杂合子的检出率差异有统计学意义(P<0.01)。结论紫外速率定量法是筛检G6PD缺乏症较理想的方法,但仍然存在一定的漏检率。更多还原

【Abstract】 Objective UV- rate quantitative method and improvement of G6 PD /6PGD ratio method,were conducted for comparative analysis and judgement,so as to establish a more effective method for the diagnosis of G6 PD deficient heterozygote.Methods Sixty G6 PD deficient heterozygote confirmed by amplification refractory mutation system( ARMS) and sixty normal patients were detected by UV- rate quantitative method and improvement of G6 PD /6PGD ratio method,respectively,and the results were compared and analyzed. Results The sensitivity and specificity of UV- rate quantitative method were 65. 0% and96. 7%,respectively,and the sensitivity and specificity of improvement of G6 PD /6 PGD ratio method were 38. 3% and 91. 7%,respectively. There was statistical significance on the difference between the two methods( P < 0. 01). Conclusion UV- rate quantitative method is an ideal method for screening of G6 PD deficient disease,though there is existence of missed detection.更多还原