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进境美国苜蓿草中截获的菊花滑刃线虫检疫鉴定
Identification of Aphelenchoides ritzemabosi in Medicago sativa imported from USA
【摘要】 2016年,天津口岸检疫人员从美国进境饲用苜蓿草中发现一种滑刃属线虫,通过形态学特征、形态测计值以及核糖体28S r RNA-D2/D3区与ITS区核酸序列比对和系统进化分析,最终确定为我国检疫性有害生物菊花滑刃线虫(Aphelenchoides ritzemabosi)。从进境饲用苜蓿草中截获该线虫为全国口岸首次。
【Abstract】 In 2016,a nematode isolate of Aphelenchoides was intercepted in Medicago sativa imported from USA in Tianjin seaport. Based on the morphological characteristics and measurements and DNA sequence analysis of ribosomal 28 S r RNA-D2/D3 and ITS regions,it was identified as A.ritzemabosi(Schwartz,1911) Steiner & Buhrer,1932 which is in the quarantine pest list of China. This is the first report for interception of A. ritzemabosi in Medicago sativa.
【关键词】 菊花滑刃线虫;
苜蓿草;
形态特征;
序列分析;
ITS;
D2/D3区;
鉴定;
【Key words】 Aphelenchoides ritzemabosi; Medicago sativa; morphological characteristics; sequence analysis; ITS; D2/D3; identification;
【Key words】 Aphelenchoides ritzemabosi; Medicago sativa; morphological characteristics; sequence analysis; ITS; D2/D3; identification;
【基金】 国家科技支撑计划项目(2012BAK11B03);天津局科技项目(2013TK072);宁波市科技创新团队(2015C110018);国家质检总局课题(2015IK023)
- 【文献出处】 植物检疫 ,Plant Quarantine , 编辑部邮箱 ,2016年05期
- 【分类号】S41-30
- 【被引频次】2
- 【下载频次】123