【摘要】 为丰富甘蔗花叶病毒(Sugarcane mosaic virus,SCMV)种群信息,探索SCMV种群结构,本研究运用RT-PCR方法克隆了采自福州的2个SCMV分离物(分别命名为FZ-C1和FZ-C2)的全基因组,结合已公布的18个SCMV分离物的全基因组序列,利用MEGA 4.1和Simplot软件对SCMV的种群结构进行了分析。结果显示,FZ-C1和FZ-C2全长分别为9 570nt和9 573 nt,均包含一个9 189 nt的开放阅读框,编码长度为3 063个氨基酸的多聚蛋白。这2个分离物与SCM V-A株系的相似性最高,酶切位点也与SCMV-A株系的基本一致。系统发育进化分析表明,SCMV可以分为3组,分别命名为SSGⅠ、Ⅱ、Ⅲ。SSGⅠ来源于玉米(Zea mays),SSGⅡ和SSGⅢ来源于甘蔗(Saccharum spp.hybrid),其中SSGⅢ主要来源于果蔗。FZ分离物聚在SSGⅡ组内。研究结果丰富了SCMV全基因组序列信息,有助于全面了解SCMV种群的遗传进化关系。更多还原

【Abstract】 This study was aimed at understanding the phylogenesis of Sugarcane mosaic virus( SCMV). The complete genomes of two SCM V isolates obtained at Fuzhou,designated as FZ-C1 and FZ-C2,respectively,were cloned in this study. Based on the genome sequences of the newSCM V isolates and published 18 SCM V isolates,the genetic diversity and population structure of SCM V were analyzed using M EGA 4.1 and Simplot softwares. Results showed that the genome of FZ-C1 contains 9 570 nucleotides,while FZ-C2 contains 9 573 nucleotides. Both FZ-C1 and FZ-C2 have a single open reading frame of 9 189 nt in length and encode a polyprotein containing 3 063 amino acids. FZ-C1 and FZ-C2 shared high similarity with SCM V-A at both nucleotide and amino acid level,as well as the restriction sites. Phylogenetic analysis showed that SCM V strains could be divi-ded into three groups: SSG I,Ⅱ and Ⅲ. Strains in SSG I were mainly isolated from Zea mays,while SSGⅡand SSG Ⅲ were from sugarcane,especially the SSG Ⅲ that were primarily isolated from chewing cane. Two FZ isolates were grouped into the SSG Ⅱ. Our work enriched the whole genome sequence information of SCM V and shed light on the phylogenesis of SCM V.更多还原