【摘要】 通过生物学特性观察和16S rDNA基因序列分析鉴定菌株SC11为壮观链霉菌(Streptomyces spectabilis)。该菌可在棉花根际土壤以及棉花根部长期定殖,30 d的定殖密度分别为1.38×105 CFU/g和2.95×103 CFU/g。2011年和2012年的大田防治试验显示,SC11菌粉对棉花枯萎病防效分别为42.51%和36.70%,与对照50%多菌灵(WP)的防效相比无显著差异,施用90~150 d的防效分别显著高于多菌灵(P<0.05)。与清水对照相比,田间施用SC11菌粉棉花倒三叶面积显著提高52.6%,籽棉和皮棉产量分别增加21.9%和23.0%(P<0.05)。研究结果表明,壮观链霉菌SC11是一株对棉花枯萎病具有应用前景的生防菌株。更多还原

【Abstract】 Based on biological property and 16 S rDNA sequence anslysis,actinomycetes strain SC11 was identified as Streptomyces spectabilis. Strain SC11 could establish a stable colonization in cotton rhizosphere and cotton root with populations of 1. 38 ×10~5 CFU /g and 2. 95 ×10~3 CFU /g 30 d after sowing,respectively. Field experiments in 2011 and 2012 showed that the powder formula of strain SC11 exhibited control efficacies( CE)of 42. 51% and 36. 70% against cotton Fusarium wilt,which was as effective as that of the 50% carbendazim( WP). During 90- 150 dpi,the CE of the strain SC11 was significantly higher than that of the carbendazim treatment control( P <0. 05). The area of the top 3 leaves treated by the SC11 increased by 52. 6% compared with the water treated control,and the yields of seed cotton and ginned cotton increased 21. 9% and 23. 0%( P< 0. 05),respectively. Results of above research suggest that the strain SC11 has a potential biocontrol ability against cotton Fusarium wilt.更多还原